Effect of exogenous sterols on the growth and fatty acid composition of the oomycete Pythium debaryanum
Effect of exogenous sterols on the growth and fatty acid composition of the oomycete Pythium debaryanum
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DOI：
10.1007/bf02756803
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发表时间：
2000-09-01
期刊：
影响因子：
1.5
通讯作者：
Konova, IV
中科院分区：
文献类型：
--
作者：
Galanina, LA;Konova, IV
研究发现外源性麦角甾醇和胆固醇会影响卵菌真菌腐霉的生长和脂肪生成，而腐霉不能从头合成类固醇化合物。这些甾醇在葡萄糖-蛋白胨培养基中的深层培养过程中刺激了真菌的生长。伴随着滞后期的缩短、活跃生长期的延长，以及响应麦角甾醇或胆固醇的添加，最大生物量分别增加3.7倍或4.3倍。在麦角甾醇存在下，细胞内多烯脂肪酸含量增加，二十碳多烯脂肪酸相对含量达到细胞内脂肪酸总量的31.4%。相反，胆固醇使细胞内多烯酸含量下降，二十碳多烯酸相对含量下降至脂肪酸总量的19.6%。可以推断，外源甾醇由于生长刺激而提高了药理活性多烯酸的产量。
Exogenous ergosterol and cholesterol were found to affect the growth and lipogenesis of the oomycete fungus Pythium debaryanum, which is unable to synthesize de novo steroid compounds. These sterols stimulated the growth of the fungus during its submerged cultivation in glucose-peptone medium. This was accompanied by the shortening of the lag phase, the lengthening of the period of active growth, and by a 3.7-or 4.3-fold increase in the maximum biomass in response to the addition of ergosterol or cholesterol, respectively. In the presence of ergosterol, the cellular content of polyenoic fatty acids increased, and the relative content of eicosapolyenoic fatty acids reached 31.4% of the total amount of fatty acids in cells. Conversely, cholesterol decreased the cellular content of polyenoic acids, and the relative content of eicosapolyenoic acids fell to 19.6% of the total amount of fatty acids. It may be inferred that exogenous sterols enhance the yield of pharmacologically active polyenoic acids because of the growth stimulation.