The use of sequential mark-release-recapture experiments to estimate population size, survival and dispersal of male mosquitoes of the Anopheles gambiae complex in Bana, a west African humid savannah village
The use of sequential mark-release-recapture experiments to estimate population size, survival and dispersal of male mosquitoes of the Anopheles gambiae complex in Bana, a west African humid savannah village
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DOI：
10.1186/s13071-017-2310-6
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发表时间：
2017-08-07
影响因子：
3.2
通讯作者：
Diabate, Abdoulaye
中科院分区：
文献类型：
--
作者：
Epopa, Patric Stephane;Millogo, Abdoul Azize;Diabate, Abdoulaye
背景：病媒控制是疟疾控制战略的一个主要组成部分。杀虫剂抗药性的日益蔓延鼓励了新工具的开发，例如利用释放的改性雄蚊进行遗传控制。使用雄蚊作为控制策略的一部分，需要更好地了解雄蚊生物学，包括影响其生存和传播的因素，以及准确估计目标蚊子种群规模的能力。本研究的目的是通过重复的标记释放-再捕获实验，以确定人口规模的季节性变化，并估计冈比亚按蚊复杂的雄性蚊子在一个小的西非village.Methods：标记释放-再捕获实验进行了连续两年，在雨季和旱季在巴纳村的生存和扩散。对于每个实验，使用三种不同颜色的染料粉末（红色、蓝色和绿色）标记约5000（3407-5273）只成年雄性科氏按蚊，并将其释放在村庄的三个不同位置（中心、边缘和外部）。释放后连续七天在村庄各处的地点重新捕获蚊子。三种不同的捕获方法：粘土罐，拟除虫菊酯喷雾捕捉和swarm sampling.Results：Swarm sampling是最有效的方法，在现场重新捕获雄蚊。通过对Fisher-Ford模型的贝叶斯分析估计了种群规模和存活率，结果表明，在旱季结束（10 000 - 50 000）至雨季（100 000 - 500 000）之间，种群规模估计数增加了约10倍。没有发现季节对蚊子生存的影响，这表明天气以外的因素可能起着重要作用。在每项研究的7天内，蚊虫的扩散范围为40至549米，不受季节的影响，但主要受释放位置的影响，这解释了净扩散距离的44%以上。这项研究清楚地表明，基于雄性的MRR实验可以用来估计野生雄性种群的一些参数，如种群大小，存活率，和扩散，并估计在一个给定的地方移动的空间模式。
Background: Vector control is a major component of the malaria control strategy. The increasing spread of insecticide resistance has encouraged the development of new tools such as genetic control which use releases of modified male mosquitoes. The use of male mosquitoes as part of a control strategy requires an improved understanding of male mosquito biology, including the factors influencing their survival and dispersal, as well as the ability to accurately estimate the size of a target mosquito population. This study was designed to determine the seasonal variation in population size via repeated mark-release-recapture experiments and to estimate the survival and dispersal of male mosquitoes of the Anopheles gambiae complex in a small west African village.Methods: Mark-release-recapture experiments were carried out in Bana Village over two consecutive years, during the wet and the dry seasons. For each experiment, around 5000 (3407-5273) adult male Anopheles coluzzii mosquitoes were marked using three different colour dye powders (red, blue and green) and released in three different locations in the village (centre, edge and outside). Mosquitoes were recaptured at sites spread over the village for seven consecutive days following the releases. Three different capture methods were used: clay pots, pyrethroid spray catches and swarm sampling.Results: Swarm sampling was the most productive method for recapturing male mosquitoes in the field. Population size and survival were estimated by Bayesian analyses of the Fisher-Ford model, revealing an about 10-fold increase in population size estimates between the end of dry season (10,000-50,000) to the wet season (100,000-500,000). There were no detectable seasonal effects on mosquito survival, suggesting that factors other than weather may play an important role. Mosquito dispersal ranged from 40 to 549 m over the seven days of each study and was not influenced by the season, but mainly by the release location, which explained more than 44% of the variance in net dispersal distance.Conclusion: This study clearly shows that male-based MRR experiments can be used to estimate some parameters of wild male populations such as population size, survival, and dispersal and to estimate the spatial patterns of movement in a given locality.