SOLUTE DISTRIBUTIONS AND TRAPPING EFFICIENCIES OBSERVED IN FREEZE-THAWED MULTILAMELLAR VESICLES
SOLUTE DISTRIBUTIONS AND TRAPPING EFFICIENCIES OBSERVED IN FREEZE-THAWED MULTILAMELLAR VESICLES
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DOI：
10.1016/0005-2736(85)90084-7
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
JANOFF, AS
中科院分区：
文献类型：
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作者：
MAYER, LD;HOPE, MJ;JANOFF, AS
干脂在含水缓冲液中的机械分散形成多层囊泡(MLV)系统并不会导致溶质的平衡跨膜分布。特别是，被捕获的缓冲液表现出较低的溶质浓度。在Mn2+存在下分散的卵磷脂酰胆碱MLV体系也表现出溶质的非平衡分布，重复冻融循环可以消除这种溶质的异质性。此外，由此产生的冻融MLV显示出极大地提高了捕获体积和捕获效率。例如，在每毫升400毫克磷脂的情况下，捕捉率可以高达90%。这与MLV形态的显著变化有关，在MLV形态中，通常可以观察到大的双层间分离。
Mechanical dispersion of dry lipid in an aqueous buffer to form multilamellar vesicle (MLV) systems does not result in equilibrium trans-membrane distributions of solute. In particular, the entrapped buffer exhibits reduced solute concentrations. Egg phosphatidylcholine MLV systems dispersed in the presence of Mn2+ also exhibit non-equilibrium solute distributions, and repetitive freeze-thawing cycles can remove such solute heterogeneity . Further, the resulting freeze-thawing MLVs exhibit dramatically enhanced trapped volumes and trapping efficiencies. At 400 mg phospholipid per ml, for example, the trapping efficiencies can be as high as 90%. This is associated with a remarkable change in MLV morphology where large inter-bilayer separations are commonly observed.