Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures
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DOI：
10.1016/s0308-8146(99)00089-8
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发表时间：
1999-09-01
期刊：
影响因子：
8.8
通讯作者：
Bast, A
中科院分区：
文献类型：
--
作者：
van den Berg, R;Haenen, GRMM;Bast, A
TEAC （Trolox等效抗氧化能力）测定是基于清除2,2'-氮唑-（3-乙基苯并噻唑-6-磺酸盐）自由基（ABTS(.-)）。在这篇报道中，我们描述了一种基于ABTS自由基阴离子预生成的修饰，用一种耐热偶氮化合物，2,2'-偶氮-（2-氨基丙烷）HCl （ABAP）。这种修改使分析不容易受到人工因素的影响，例如对自由基生成过程的影响。对于大多数抗氧化剂测试，一个双相反应模式被看到，即快速和缓慢的清除率。我们对具有抗氧化能力的亲脂性和亲水性化合物进行了评价。对几种与水相容的有机溶剂进行了α -生育酚、槲皮素和β -胡萝卜素的测试。发现teac在不同溶剂中存在差异。在标准化条件下，可以证明从单个抗氧化剂中得到的teac的可加性。这可能使该测定法应用于鉴定“未知”抗氧化剂。1999爱思唯尔科学有限公司版权所有。
The TEAC (Trolox equivalent antioxidant capacity) assay is based on scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) radical anions (ABTS(.-)). In this report we describe a modification based on pre-generation of the ABTS radical anions with a thermolabile azo compound, 2,2'-azobis- (2-amidinopropane)HCl (ABAP). This modification makes the assay less susceptible to artefacts, e.g. influence on the radical generation process. For most antioxidants tested, a biphasic reaction pattern was seen, i.e. a fast and slow scavenging rate. We evaluated application of the assay with both lipophilic and hydrophilic compounds with antioxidant capacity. Several organic solvents, compatible with water, were tested with alpha-tocopherol, quercetin and beta-carotene. It was found that the TEACs differed in various solvents. Under standardized conditions additivity of TEACs obtained from individual antioxidants could be demonstrated. This might enable application of the assay for the identification of "unknown" antioxidants. (C) 1999 Elsevier Science Ltd. All rights reserved.