Associations of UBE21 with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system
Associations of UBE21 with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system
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DOI：
10.1006/geno.1996.0540
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发表时间：
1996-10-15
期刊：
影响因子：
4.4
通讯作者：
Chen, DJ
中科院分区：
文献类型：
--
作者：
Shen, ZY;PardingtonPurtymun, PE;Chen, DJ
酵母RAD52依赖性途径参与DNA重组和双链断裂修复。酵母泛素结合酶UBCS参与S期和M期细胞周期蛋白降解和有丝分裂控制。在酵母双杂交系统中使用人RAD 52蛋白作为“诱饵"，我们已经鉴定了酵母UBCS的人同源物，命名为UBE 2I，其与RAD 52、RAD 5I、p53和泛素样蛋白UBL 1相互作用。这些相互作用是UBE2I特异性的，因为另一种与DNA修复相关的泛素缀合酶RAD6（UBC 2）不与这些蛋白质相互作用。UBE 2I与RAD52的相互作用是由RAD52的自结合区介导的。这些结果表明，RAD52依赖的过程，细胞周期控制，p53介导的途径，和泛素化通过人UBE2I相互作用。(C)出版社：Academic Press，Inc.
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBCS participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the ''bait'' in a yeast two-hybrid system, we have identified a human homolog of yeast UBCS, designated UBE2I, that interacts with RAD52, RAD5I, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I. (C) 1996 Academic Press, Inc.