HUMAN IMMUNODEFICIENCY VIRUS MAY ENCODE A NOVEL PROTEIN ON THE GENOMIC DNA PLUS STRAND
HUMAN IMMUNODEFICIENCY VIRUS MAY ENCODE A NOVEL PROTEIN ON THE GENOMIC DNA PLUS STRAND
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DOI：
10.1126/science.3347840
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发表时间：
1988-03-18
期刊：
影响因子：
56.9
通讯作者：
MILLER, RH
中科院分区：
文献类型：
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作者：
MILLER, RH
已知人类免疫缺陷病毒 (HIV) 的基因组在双链 DNA 复制中间体的负链上包含 8 个开放阅读框 (ORF)。这里提供的数据表明，HIV 的 DNA 正链在与包膜基因序列互补的区域中包含一个先前未识别的 ORF。如果翻译从第一个起始密码子开始，这个 ORF 可以编码大约 190 个氨基酸残基的蛋白质，相对分子质量为 20 千道尔顿。预测的蛋白质具有高度疏水性，因此可能与膜相关。因此，HIV 基因组有可能编码反义信使 RNA 上的蛋白质。
The genome of the human immunodeficiency virus (HIV) is known to contain eight open reading frames (ORFs) on the minus strand of the double-stranded DNA replicative intermediate. Data presented here indicate that the DNA plus strand of HIV contains a previously unidentified ORF in a region complementary to the envelope gene sequence. This ORF could encocde a protein of approximately 190 amino acid residues with a relative molecular mss of 20 kilodaltons if translation began from the first initiation codon. The predicted protein is highly hydrophobic and thus could be membrane associated. It is possible, therefore, that the HIV genome encodes a protein on antisense messenger RNA.