[Electroacupuncture intervention relieves pain possibly by promoting MOR endocytosis in locus coeruleus in bone cancer pain rats with morphine tolerance].
[Electroacupuncture intervention relieves pain possibly by promoting MOR endocytosis in locus coeruleus in bone cancer pain rats with morphine tolerance].
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DOI：
10.13702/j.1000-0607.180635
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发表时间：
2019-03-25
期刊：
影响因子：
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通讯作者：
Liang, Yi
中科院分区：
文献类型：
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作者：
Shi, Ren-Yi;Fu, Tao-Fang;Liang, Yi
目的：观察电针(EA)对吗啡耐受(MT)骨癌痛(BCP)大鼠疼痛行为及蓝斑区-阿片受体(MOR)和-阿片受体内化重要蛋白分子Rab5表达的影响，探讨其改善BCP和MT的作用机制。第一部分将23只雌性SD大鼠随机分为假BCP组(n=6)、BCP组(n=9)和BCP+MT组(n=8)；第二部分将61只雌性SD大鼠随机分为5组：假BCP组(n=11)、BCP组(n=11)、BCP+MT组(n=13)、BCP+MT+EA组(n=13)和BCP+MT+Sham EA组(n=13)。采用左侧胫骨上段骨髓腔内注射10 L人Walker256乳腺癌细胞(MRMT-1乳腺癌细胞，1×104个/L)和10 mg/kg盐酸吗啡(10 mg/kg，每12h 1次，连续11d)的方法建立大鼠骨髓腔内吗啡耐受模型。接种后第21天，首次腹腔注射吗啡后，立即开始对双侧“足三里”和“昆仑”进行电针(2 Hz/100 Hz，0.5~1.5 mA，每10min增加0.5 mA)。治疗30min/次，每日1次，连续7d。分别于接种前、接种后第10、11、21、22、24、26、28天检测小鼠的缩足阈值(PWT)。结果：在实验的第一部分，在肿瘤细胞接种后第10天，BCP组和BCP+MT组的PWT明显低于假BCP组(P
OBJECTIVE: To observe the effect of electroacupuncture (EA) on pain behavior and expression of -opioid receptor (MOR) and Rab5 (an important protein molecule for internalization of MOR) in the locus coeruleus (LC) region in bone cancer pain (BCP) rats with morphine tolerance (MT), so as to explore its mechanisms underlying improvement of BCP and MT.METHODS: The present study included two parts. In the first part, 23 female SD rats were randomized into sham BCP (n＝6), BCP (n＝9) and BCP＋MT (n＝8) groups, and in the second part, 61 female SD rats were randomized into 5 groups: sham BCP (n＝11), BCP (n＝11), BCP＋MT (n＝13), BCP＋MT＋EA (n＝13) and BCP＋MT＋sham EA (n＝13). The BCP morphine tolerance (BCP＋MT) model was established by injection of 10 L of human Walker 256 breast cancer cells (MRMT-1 breast cancer cells, 1 x104 cells/L) into the bone marrow cavity at the upper part of the left tibia and intraperitoneal injection of morphine hydrochloride (10 mg/kg, once per 12 h, for 11 successive days). On day 21 after inoculation, EA (2 Hz/100 Hz, 0.5－1.5 mA, increasing 0.5 mA every 10 min) was began to applied to bilateral "Zusanli" (ST30) and "Kunlun" (BL60) immediately after the first intraperitoneal injection of morphine. The treatment was performed for 30 min every time, once daily for 7 successive days. The paw withdrawal threshold (PWT) was detected before and 10, 11, 21, 22, 24, 26 and 28 days after inoculation. The immunoactivity of MOR and Rab5 proteins in the LC region was detected by immunofluorescence histochemistry.RESULTS: In the first part of the study, at the 10th day after inoculation of cancer cells, the PWT of the BCP and BCP＋MT groups was significantly lower than that of the sham BCP group (P