Sex differences in hepatic cytochrome P-450 monooxygenase activities in rainbow trout during an annual reproductive cycle.
Sex differences in hepatic cytochrome P-450 monooxygenase activities in rainbow trout during an annual reproductive cycle.
复制标题
每年繁殖周期期间虹鳟鱼肝细胞色素 P-450 单加氧酶活性的性别差异。
作者：
L. Förlin;C. Haux
研究了虹鳟鱼肝微体细胞色素P-450单加氧酶活性。这些鱼被饲养在10摄氏度的恒温下供应淡水的水箱中，每天的光和暗周期被调整以跟随自然光周期。每个月采样一次，为期1年。在虹鲑性发育后期，苯并(A)芘羟基酶(或芳烃羟基酶；AHH)、乙氧基香豆素-O-脱乙基酶(ECOD)和乙基吗啡-N-脱甲基酶(END)活性和细胞色素P-450含量较高。当单加氧酶活性在每个细胞色素P-450的基础上表达时，仅观察到AHH和ECOD活性的性别差异。由此发现，END的性别变异与细胞色素P-450的总量密切相关。结果表明，虹鱼肝脏细胞色素P-450同工酶存在性别差异。血浆雌二醇和睾酮水平的年度模式与细胞色素P-450单加氧酶的性别相关差异的相似性支持了性类固醇在调节细胞色素P-450系统中发挥作用的论点。
Hepatic microsomal cytochrome P-450 monooxygenase activities were investigated in rainbow trout during an annual reproductive cycle. The fish were kept in tanks supplied with fresh water at a constant temperature of 10 degrees C. The daily light and darkness cycle was adjusted to follow the natural photoperiod. Sampling was performed once every month for 1 year. Higher benzo(a)pyrene-hydroxylase (or aryl hydrocarbon hydroxylase; AHH), ethoxycoumarin-O-deethylase (ECOD) and ethylmorphine-N-demethylase (END) activities and cytochrome P-450 content were found during the late stage of sexual development in rainbow trout. When monooxygenase activities were expressed on a per cytochrome P-450 basis, sex-dependent differences were observed only for AHH and ECOD activities. It was thus found that sex-dependent variations of END were closely correlated with the total amount of cytochrome P-450. The results indicate that differences exist in hepatic cytochrome P-450 isoenzyme patterns between the sexes in rainbow trout. The similarity of the annual pattern of plasma levels of oestradiol and testosterone to that of sex-dependent differences in the cytochrome P-450 monooxygenases support the contention that sex steroids play a role in regulating the cytochrome P-450 system.