Flagellar quiescence response in sea urchin sperm induced by electric stimulation.
Flagellar quiescence response in sea urchin sperm induced by electric stimulation.
复制标题
电刺激诱导海胆精子鞭毛静止反应。
DOI：
10.1002/cm.970310107
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发表时间：
1995
影响因子：
--
通讯作者：
K. Takahashi
中科院分区：
文献类型：
--
作者：
C. Shingyoji;K. Takahashi
为了研究精子鞭毛静止的机制，我们研究了电刺激单个海胆（Hemicentrotus pulcherrimus）精子的效果。用吸附电极刺激精子头部引起鞭毛静止反应，其中当电极用作阳极时，精子在鞭毛的近端区域显示出典型的手杖形弯曲。阴极刺激也诱导静止，但远不如阳极刺激有效。在持续1-3 s的静止反应期间，没有新的弯曲开始，随后鞭毛恢复正常跳动。在海水中，Ca 2+（> 2 mM）对该反应有抑制作用，而Co2+和La 3+则对该反应有抑制作用。在低Ca 2+浓度（2-5 mM）时，藤形弯曲的角度小于10 mM Ca 2+时的角度;因此，藤形弯曲的角度，静止响应的特征取决于Ca 2+浓度。这些结果表明，平静反应是由鞭毛膜的去极化，然后通过Ca 2+通道进入鞭毛内的Ca 2+的流入触发。鞭毛内Ca 2+浓度的增加影响滑动量，从而产生各种角度的手杖形近端弯曲，同时抑制近端弯曲（主弯曲）的传播和新的反向弯曲的形成。
To investigate the mechanism of the flagellar quiescence in sperm, we examined the effect of electric stimulation of individual spermatozoa of the sea urchin, Hemicentrotus pulcherrimus. Stimulation with a suction electrode attached to the sperm head elicited a flagellar quiescence response, in which the sperm showed a typical cane-shaped bend in the proximal region of the flagellum when the electrode was used as anode. Cathodic stimulation also induced quiescence, but was much less effective than anodic stimulation. During the quiescence response, which lasted for 1-3 s, no new bend was initiated, and subsequently the flagellum resumed normal beating. The quiescence response required the presence of Ca2+ (> 2 mM) in sea water, and was inhibited by Co2+ and La3+. At low Ca2+ concentrations (2-5 mM), the angle of the cane-shaped bend was smaller than that at 10 mM Ca2+; thus the angle of the cane-shaped bend, characteristic of the quiescence response is dependent on Ca2+ concentration. These results suggest that the quiescence response is triggered by a depolarization of the flagellar membrane, followed by an influx of Ca2+ into the flagellum through Ca2+ channels. The increase in Ca2+ concentration within the flagellum affects the amount of sliding and thus produces a cane-shaped proximal bend of various angles, while inhibiting both the propagation of the proximal bend (principal bend) and the formation of a new reverse bend.
Ca2+扩散与海胆精子鞭毛的静止反应。
影响因子：
2
作者：
Blum,JJ;Hines,MH
通讯作者：
Hines,MH