Expression of c-kit receptor in normal and transformed human nonlymphoid tissues.
Expression of c-kit receptor in normal and transformed human nonlymphoid tissues.
复制标题
c-kit 受体在正常和转化的人类非淋巴组织中的表达。
作者：
P. Natali;M. Nicotra;I. Sures;E. Santoro;A. Bigotti;A. Ullrich
原癌基因c-kit编码分子量为145,000的酪氨酸激酶，与血小板衍生生长因子/集落刺激因子受体高度相关。小鼠基因突变会导致造血、配子发生和黑素细胞谱系受损。为了阐明 c-kit 在发育和肿瘤发生中的功能，我们通过免疫组织化学方法分析了其在人类正常和转化的非淋巴组织中的表达。该受体已在精原细胞、黑素细胞中检测到，并且出人意料地在星形胶质细胞、肾小管、腮腺细胞、甲状腺细胞和乳腺上皮中也被检测到。虽然该基因产物在精原细胞瘤、肺肿瘤和低侵袭性黑色素瘤中表达，但在甲状腺癌和乳腺癌、星形细胞瘤和侵袭性黑色素瘤中未检测到可检测水平。在乳腺肿瘤中，这些发现通过对正常组织和转化组织的 RNA 制剂进行配对 Northern 印迹分析得到了证实。目前的结果表明，c-kit 受体在更大范围的细胞谱系的发育中发挥着作用。此外，根据转化相关的变化，我们推测，虽然在某些细胞类型中，c-kit表达正向调节有丝分裂发生并在肿瘤转化中被选择，但在其他组织中，c-kit途径参与形态发生和分化，因此在肿瘤进展过程中被负向选择。
The protooncogene c-kit encodes a tyrosine kinase with a molecular weight of 145,000, highly related to the platelet derived growth factor/colony stimulating factor receptors. Mutations of the murine gene result in impairment of hematopoiesis, gametogenesis, and of the melanocyte cell lineage. In order to elucidate c-kit functions in development and oncogenesis we have analyzed immunohistochemically its expression in human normal and transformed nonlymphoid tissues. The receptor has been detected in spermatogonia, melanocytes, and unexpectedly, in astrocytes, renal tubules, parotid cells, thyrocytes, and breast epithelium. While the gene product is expressed in seminoma, lung tumors, and melanoma of low invasiveness, no detectable levels have been detected in thyroid and breast carcinomas, astrocytomas, and invasive melanomas. In breast tumors these findings were confirmed by paired, Northern blot analysis of RNA preparations from normal and transformed tissue. The present results demonstrate that the c-kit receptor plays a role in the development of a larger spectrum of cell lineages. Furthermore, on the basis of the transformation associated changes, we speculate that, while in some cell types, c-kit expression positively regulates mitogenesis and is selected for in neoplastic transformation, in other tissues the c-kit pathway is involved in morphogenesis and differentiation and is, therefore, negatively selected in the course of tumor progression.
影响因子：
2.9
作者：
Sumbele S;Fotelli MN;Nikolopoulos D;Tooulakou G;Liakoura V;Liakopoulos G;Bresta P;Dotsika E;Adams MA;Karabourniotis G
通讯作者：
Karabourniotis G
ras原癌基因蛋白在正常人体组织中的表达。
影响因子：
8
作者：
Furth,ME;Aldrich,TH;Cordon-Cardo,C
通讯作者：
Cordon-Cardo,C