Functionalization of Polymeric Nanofilter Biointerface for Small Biomarker Sensing
Functionalization of Polymeric Nanofilter Biointerface for Small Biomarker Sensing
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用于小生物标志物传感的聚合物纳米过滤器生物界面的功能化
DOI：
10.1149/09706.0009ecst
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sakata Toshiya
中科院分区：
文献类型：
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作者：
Fukuma Toru;Nishitani Shoichi;Sakata Toshiya
我们提出了一种聚合物纳米过滤器生物界面与多层抑制非特异性信号所造成的干扰物种，如蛋白质和小生物分子的电位生物传感器。在以前的工作中，我们成功地防止小生物分子与Au电极反应，使用具有单个聚合物层的聚合物纳米过滤器，导致在扩展Au栅场效应晶体管（FET）中抑制非特异性信号。在这项研究中，聚（2-甲基丙烯酰氧乙基磷酰胆碱）（PMPC）是另外采用作为一个纳米过滤层，以抑制蛋白质的非特异性吸附在Au栅极表面。我们通过重氮化学和电子转移表面引发原子转移自由基聚合（ARGET SI-ATRP）再生的活化剂将聚合物纳米过滤器精确地接枝到Au电极上。通过阻抗分析，我们发现具有PMPC基纳米过滤器的Au电极的电荷转移电阻（RCT）几乎没有变化，即使当人血清白蛋白（HSA）添加到电极上。也就是说，基于PMPC的纳米过滤器有效地有助于防止蛋白质如HSA的非特异性吸附。
We propose a polymeric nanofilter biointerface with a multilayer that suppresses nonspecific signals caused by interfering species such as proteins and small biomolecules for potentiometric biosensors. In a previous work, we successfully prevented small biomolecules from reacting with the Au electrode using a polymeric nanofilter with a single polymeric layer, resulting in the suppression of nonspecific signals in an extended-Au-gate field-effect transistor (FET). In this study, poly (2-methacryloyloxyethyl phosphorylcholine)(PMPC) is additionally employed as an antifouling nanofilter layer to suppress the nonspecific adsorption of proteins on the Au gate surface. We precisely graft the polymeric nanofilter on the Au electrode via diazonium chemistry and activators regenerated by electron transfer surface-initiated atom transfer radical polymerization (ARGET SI-ATRP). By impedimetric analysis, we found that the charge transfer resistance (RCT) of the Au electrode with the PMPC-based nanofilter hardly changes even when human serum albumin (HSA) is added onto the electrode. That is, the PMPC-based nanofilter effectively contributes to the prevention of the nonspecific adsorption of proteins such as HSA.