Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant
Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant
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DOI：
10.1128/aem.63.4.1344-1348.1997
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发表时间：
1997-04-01
影响因子：
4.4
通讯作者：
Romero, J
中科院分区：
文献类型：
--
作者：
Espejo, RT;Romero, J
由于连续添加硫酸和回收浸出液，大多数铜生物浸出厂的硫酸盐浓度很高。由于参与生物浸出的细菌通常在低硫酸盐浓度下分离，因此对铜生产厂使用的高硫酸盐溶液（1.25 M）浸出的矿石中的细菌种群进行了调查，通过对从浸出矿石中提取的 DNA 进行 PCR 扩增后观察到的 16S 和 23S rRNA 基因之间间隔区的长度模式来评估原始种群的复杂性，通过电泳迁移区分了 6 个主要间隔区，但它们可以进一步解析为 8 个。间隔区通过核苷酸序列同源性来确定，同源性程度由杂交后形成的异源双链体的电泳迁移推断。其中一个间隔区与氧化硫硫杆菌中发现的间隔区无法区分，四个可能与氧化亚铁硫杆菌有关，三个可能与氧化亚铁钩端螺旋菌有关。在含有不同无机能源的培养基中培养后，原始样品中只有五个间隔物可以恢复。总而言之，结果表明浸出矿石中的细菌形成了一个由至少三个物种组成的群落：相当同质的氧化硫硫杆菌菌株和氧化亚铁杆菌和氧化亚铁乳杆菌菌株的两个异质种群。
Most copper bioleaching plants operate with a high concentration of sulfate salts caused by the continuous addition of sulfuric acid and the recycling of the leaching solution. Since the bacteria involved in bioleaching have been generally isolated at low sulfate concentrations, the bacterial population in ores leached with the high-sulfate solution (1.25 M) employed in a copper production plant was investigated, The complexity of the original population was assessed by the length pattern of the spacer regions between the 16S and 23S rRNA genes, observed after PCR amplification of the DNA extracted from the leached ore, Six main spacers were distinguished by electrophoretic migration, but they could be further resolved into eight spacers by nucleotide sequence homology, The degree of homolog was inferred from the electrophoretic migration of the heteroduplexes formed after hybridization. One of the spacers was indistinguishable from that found in Thiobacillus thiooxidans, four could be related to Thiobacillus ferrooxidans, and three could be related to Leptospirillum ferrooxidans. Only five of the spacers in the original sample could be recovered after culturing in media containing different inorganic energy source. Altogether, the results indicate that the bacteria in the leached ore formed a community composed of at least three species: a fairly homogeneous population of T. thiooxidans strains and two heterogeneous populations of T. ferrooxidans and L. ferrooxidans strains.