The Gut Bacteria Associated with Camponotus japonicus Mayr with Culture-Dependent and DGGE Methods
The Gut Bacteria Associated with Camponotus japonicus Mayr with Culture-Dependent and DGGE Methods
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DOI：
10.1007/s00284-012-0197-1
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发表时间：
2012-08
影响因子：
2.6
通讯作者：
Xiao-Peng Li;Xiaoning Nan;Cong Wei;Hong He
中科院分区：
文献类型：
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作者：
Xiao-Peng Li;Xiaoning Nan;Cong Wei;Hong He
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The bacterial composition and distribution in the different gut regions ofCamponotus japonicuswere investigated using both culture-dependent method and culture-independent method of polymerase chain reaction and denaturing gradient gel electrophoresis (PCR–DGGE). Five different bacterial strains were isolated using culture-dependent method, and they all belong to the phylum Firmicutes, including three genera of bacteriaBacillus,Paenibacillus,andEnterococcus.Bacillus cereusandEnterococcus mundtiiwere found in the midgut;Paenibacillussp. was isolated from the hindgut; and the other twoBacillusspp. were isolated from the crop. Twelve distinct DGGE bands were found using PCR–DGGE method, and their sequences blasting analysis shows that they are members of the Proteobacteria and the Firmicutes, respectively, including three genera (Pseudomonas,CandidatusBlochmannia,Fructobacillus) and one uncultured bacterium, in whichPseudomonaswas the most dominant bacteria group in all the three gut regions. According to the DGGE profile, the three gut regions had very similar gut communities, and all the DGGE bands were presented in the midgut and hindgut, while just two bands representingBlochmanniawere not present in the crop. The results of our study indicate that the gut ofC. japonicusharbors several other bacteria besides the obligate endosymbiontsBlochmannia,and more work should be carried on to verify if they are common in the guts of otherCamponotusants.