Serum anti-BPAG1 auto-antibody is a novel marker for human melanoma.
Serum anti-BPAG1 auto-antibody is a novel marker for human melanoma.
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DOI：
10.1371/journal.pone.0010566
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发表时间：
2010-05-10
期刊：
影响因子：
3.7
通讯作者：
Kaneda Y
中科院分区：
文献类型：
--
作者：
Shimbo T;Tanemura A;Yamazaki T;Tamai K;Katayama I;Kaneda Y
恶性黑色素瘤是最具侵袭性的肿瘤类型之一。由于恶性黑色素瘤一旦转移就很难治疗，因此早期发现和治疗至关重要。因此，寻找早期黑色素瘤的可靠生物标志物受到了广泛关注。通过使用一种新的筛选肿瘤抗原及其自身抗体的方法，我们确定了大疱性类天疱疮抗原1（BPAG 1）作为其自身抗体识别的黑色素瘤抗原。BPAG 1是皮肤病大疱性类天疱疮（BP）中的自身抗原，并且抗BPAG 1自身抗体可在来自BP患者的血清中检测到并用于BP诊断。然而，BPAG1一直被认为是主要的角质形成细胞相关蛋白和BPAG1表达和黑色素瘤之间的关系还没有以前的报道。在本研究中，我们表明，bpag1表达在小鼠F10黑色素瘤细胞系在体外和F10黑色素瘤肿瘤在体内和BPAG 1表达在人类黑色素瘤细胞系（A375和G361）和正常的人类黑色素细胞。此外，黑素瘤患者血清中的抗BPAG 1自身抗体水平显著高于健康志愿者血清中的水平（p<0.01）。此外，在疾病的早期和晚期阶段的黑素瘤患者中检测到抗BPAG1自身抗体。在这里，我们报告抗BPAG1自身抗体作为诊断黑色素瘤的一个有前途的标志物，我们讨论了这种自身抗体的检测在癌症生物学和患者的意义。
Malignant melanoma is one of the most aggressive types of tumor. Because malignant melanoma is difficult to treat once it has metastasized, early detection and treatment are essential. The search for reliable biomarkers of early-stage melanoma, therefore, has received much attention. By using a novel method of screening tumor antigens and their auto-antibodies, we identified bullous pemphigoid antigen 1 (BPAG1) as a melanoma antigen recognized by its auto-antibody. BPAG1 is an auto-antigen in the skin disease bullous pemphigoid (BP) and anti-BPAG1 auto-antibodies are detectable in sera from BP patients and are used for BP diagnosis. However, BPAG1 has been viewed as predominantly a keratinocyte-associated protein and a relationship between BPAG1 expression and melanoma has not been previously reported. In the present study, we show that bpag1 is expressed in the mouse F10 melanoma cell line in vitro and F10 melanoma tumors in vivo and that BPAG1 is expressed in human melanoma cell lines (A375 and G361) and normal human melanocytes. Moreover, the levels of anti-BPAG1 auto-antibodies in the sera of melanoma patients were significantly higher than in the sera of healthy volunteers (p<0.01). Furthermore, anti-BPAG1 auto-antibodies were detected in melanoma patients at both early and advanced stages of disease. Here, we report anti-BPAG1 auto-antibodies as a promising marker for the diagnosis of melanoma, and we discuss the significance of the detection of such auto-antibodies in cancer biology and patients.
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影响因子：
13.6
作者：
Oyarbide-Valencia, Kepa;van den Boorn, Jasper G.;Le Poole, I. Caroline
通讯作者：
Le Poole, I. Caroline
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作者：
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作者：
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通讯作者：
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作者：
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