The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro.
The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro.
复制标题
基质胶和神经营养因子的三维培养系统在体外保留螺旋神经节神经元的结构和功能
DOI：
10.1155/2016/4280407
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发表时间：
2016
影响因子：
3.1
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Sun G;Liu W;Fan Z;Zhang D;Han Y;Xu L;Qi J;Zhang S;Gao BT;Bai X;Li J;Chai R;Wang H
螺旋神经节全器官培养是一种便于对活体螺旋神经节神经元(SGN)进行操作和分析的模型系统。三维(3D)培养已被证明具有许多生物医学应用，但3D培养在维持外植体培养中SGN结构和功能方面的作用尚不清楚。在这项研究中，我们使用Matrigel包裹从新生小鼠分离的螺旋神经节区域。首先，我们对3D培养体系中的基质浓度进行了优化，发现3D培养体系保护了神经节细胞的凋亡，保护了螺旋神经节区的结构，促进了神经节突起的萌发和突起生长。其次，我们发现3D培养系统促进了生长锥的生长，这体现在丝状孢子的平均数量和平均长度以及生长锥面积都较大。3D培养系统也显著增加了SGN的突触密度。最后，我们发现3D培养系统联合神经营养因子对神经突起生长的促进作用明显优于3D培养组和单纯神经营养因子处理组。综上所述，3D培养系统在外植体培养中保留了SGN的结构和功能。
Whole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the SGNs structure and function in explant culture remains uninvestigated. In this study, we used the matrigel to encapsulate the spiral ganglion region isolated from neonatal mice. First, we optimized the matrigel concentration for the 3D culture system and found the 3D culture system protected the SGNs against apoptosis, preserved the structure of spiral ganglion region, and promoted the sprouting and outgrowth of SGNs neurites. Next, we found the 3D culture system promoted growth cone growth as evidenced by a higher average number and a longer average length of filopodia and a larger growth cone area. 3D culture system also significantly elevated the synapse density of SGNs. Last, we found that the 3D culture system combined with neurotrophic factors had accumulated effects in promoting the neurites outgrowth compared with 3D culture or NFs treatment only groups. Together, we conclude that the 3D culture system preserves the structure and function of SGN in explant culture.