Humoral regulation of monocytopoiesis during the early phase of an inflammatory reaction caused by particulate substances.
Humoral regulation of monocytopoiesis during the early phase of an inflammatory reaction caused by particulate substances.
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颗粒物质引起的炎症反应早期阶段单核细胞生成的体液调节。
DOI：
10.1182/blood.v50.1.141.141
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发表时间：
1977
期刊：
影响因子：
20.3
通讯作者：
R. van Furth
中科院分区：
文献类型：
--
作者：
D. van Waarde;E. Hulsing;L. Sandkuyl;R. van Furth
腹膜内注射各种颗粒物质（即，二氧化硅、高岭土和聚苯乙烯乳胶）进入瑞士小鼠体内可引起腹膜腔中的炎性反应，伴随外周血中的单核细胞增多。在这种反应的早期阶段，在血清中证明了引起单核细胞生成增加（EMT）的因子。在注射乳胶或高岭土后18小时和注射二氧化硅后18-48小时收集的血清中发现最高的抗氧化活性;在后一种情况下，抗氧化活性可被证明长达144小时。腹膜内注射二氧化钛或盐水后）。在炎症反应开始消退后，也不能证明炎症，即，从胶乳注射后48小时开始和二氧化硅注射后192小时开始。小鼠静脉注射异丙肾上腺素后，由于外周血淋巴细胞和粒细胞的数量仅显示少量增加或根本没有增加，因此特异性地诱发单核细胞增多症，在48小时达到峰值（正常值的2.5-3倍）。在最初的24小时内，由于前单核细胞的数量几乎增加了两倍，并且这些细胞的细胞周期时间减少（从13.0小时减少到9.8小时），因此，骨髓中单核细胞的产量增加了一倍。在正常小鼠的腹膜细胞提取物中可检测到β-内酰胺酶。在诱导炎症反应后，这些提取物的活性迅速降低，并在炎症反应消退时恢复。这一过程与这些小鼠血清中的β-内酰胺酶活性相反。
An intraperitoneal injection of various particulate substances (i.e., silica, kaolin, and polystyrene latex) into Swiss mice can cause an inflammatory reaction in the peritoneal cavity with concomitant monocytosis in the peripheral blood. During the early phase of such a reaction, a factor causing increased monocytopoiesis (FIM) was demonstrated in the serum. The highest FIM activity was found in sera collected 18 hr after the injection of latex or kaolin, and 18-48 hr after the injection of silica; in the latter case, FIM could be demonstrated up to 144 hr. FIM could not be demonstrated in the serum of normal untreated mice or mice showing no or virtually no inflammatory reaction (i.e., after the intraperitoneal injection of titanium dioxide or saline). FIM also could not be demonstrated after the inflammatory reaction began to subside, i.e., from 48 hr onward after latex and 192 hr after silica injection. The intravenous injection of FIM in mice evoked specifically a monocytosis with a peak at 48 hr (2.5-3 times normal values), since the numbers of peripheral blood lymphocytes and granulocytes showed only small increases or none at all. FIM doubled the production of monocytes in the bone marrow during the first 24 hr owing to an almost twofold increase in the number of promonocytes and a decrease in the cell-cycle time (from 13.0 to 9.8 hr) of these cells. FIM was detectable in extracts of peritoneal cells of normal mice. After induction of an inflammatory reaction the activity of these extracts diminishes rapidly and is restored when the inflammatory reaction subsides. This course is the reverse of that of the FIM activity in the sera of these mice.