STRAIN VARIATION AND NUCLEAR-ASSOCIATION OF NEWCASTLE-DISEASE VIRUS MATRIX PROTEIN
STRAIN VARIATION AND NUCLEAR-ASSOCIATION OF NEWCASTLE-DISEASE VIRUS MATRIX PROTEIN
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DOI：
10.1128/jvi.62.2.586-593.1988
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发表时间：
1988-02-01
影响因子：
5.4
通讯作者：
PEEPLES, ME
中科院分区：
文献类型：
--
作者：
FAABERG, KS;PEEPLES, ME
制备了5株抗纽卡斯尔病毒（NDV）澳大利亚-维多利亚（AV）株基质（M）蛋白的单克隆抗体，并对其进行了鉴定。在竞争性抗体结合测定中，抗体分为三个离散的组。将这些抗体组描述的抗原位点命名为M1、M2和M3。每种抗体以其组特有的方式与一组NDV毒株反应，通过竞争性抗体结合证实了分组。在所有12个供试毒株上仅发现M1位点，可能是“pan-NDV”表位。所有五种单克隆抗体均在感染细胞的细胞核中检测到AV株的大部分M蛋白。此外，抗体仅染色用表达含有相应抗原位点的M蛋白的NDV毒株感染的细胞的细胞核。这些结果证实了细胞核中的免疫反应性实际上是由M蛋白引起的，而不是由病毒感染诱导的交叉反应宿主蛋白引起的。
Five monoclonal antibodies to the matrix (M) protein of Newcastle disease virus (NDV) Australia-Victoria (AV) strain were generated and characterized. In competitive antibody-binding assays, the antibodies fell into three discrete groups. The antigen sites described by these antibody groups were designated M1, M2, and M3. Each antibody reacted with a panel of NDV strains in a manner unique to its group, confirming the grouping by competitive antibody binding. Only site M1 was found on all 12 of the strains tested and may be a "pan-NDV" epitope. A large portion of the M protein of strain AV was detected in the nuclei of infected cells by all five monoclonal antibodies. In addition, the antibodies only stained the nuclei of cells infected with NDV strains expressing M protein containing the corresponding antigenic site. These results confirm that the immunoreactivity in the nucleus is actually caused by the M protein and not by a cross-reacting host protein induced by viral infection.