Allelic Loss of the ING Gene Family Loci Is a Frequent Event in Ameloblastoma
Allelic Loss of the ING Gene Family Loci Is a Frequent Event in Ameloblastoma
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DOI：
10.3727/096504010x12704916124864
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Nagatsuka, Hitoshi
中科院分区：
文献类型：
--
作者：
Borkosky, Silvia S.;Gunduz, Mehmet;Nagatsuka, Hitoshi
成釉细胞瘤是最常见的牙源性肿瘤，其特点是局部侵袭性，复发率高。虽然很少见转移能力肿瘤抑制基因（TSG）的丧失或失活允许细胞获得肿瘤生长ING家族蛋白是肿瘤抑制因子，其在物理上和功能上与p53相互作用以在细胞凋亡中发挥重要作用。TP 53基因在成釉细胞瘤中很少发生改变，因此我们将研究的重点放在ING家族基因上，分析了ING家族基因的杂合性丢失（洛）情况。（ING 1-ING 5）位点的杂合性缺失（ING洛）与成釉细胞瘤临床病理特征的关系。ING基因家族成员的每个位点均存在高频率的洛缺失（33.3 ~ 72.2%）D2 S140位点的洛缺失率与D2 S140位点的杂合性有显著相关性（P <0.05）。（ING 5基因座）和实体瘤类型（p = 0.02）ING 3 MS的洛缺失（ING 3基因座）在实体型肿瘤中也很高，显示出接近显著的相关性。此外，ING家族基因的洛在实体性成釉细胞瘤中是一种常见的遗传变异，本研究提供了关于等位基因丢失的潜在预后意义的有趣的新信息。ING基因家族位点在成釉细胞瘤发生中的作用
Ameloblastoma is the most frequently encountered odontogenic tumor, characterized by a locally invasive behavior, frequent recurrences, and. although rare. metastatic capacity Loss or inactivation of tumor suppressor genes (TSGs) allows cells to acquire neoplasm growth The ING family proteins are tumor suppressors that physically and functionally interact with p53 to perform important roles in apoptosis. DNA repair, cell cycle regulation, and senescence TP53 genetic alterations were reported to infrequently occur in ameloblastoma Considering that other TSGs related to TP53 could be altered in this tumor, we focused our study on the ING family genes We analyzed the loss of heterozygosity (LOH) status of the ING family (ING1-ING5) chromosomal loci in a group of ameloblastomas by microsatellite analysis, and correlated the ING LOH status with clinicopathological characteristics By using specific microsatellite markers, high frequency of LOH was found at the loci of each ING gene family member (33 3-72.2%) A significant relationship was shown between LOH of D2S140 (ING5 locus) and solid tumor type (p = 0.02) LOH of ING3MS (ING3 locus) was also high in solid type tumors, showing a near significant association In addition, a notable tendency toward higher LOH for half of the markers was observed in recurrent cases LOH of ING family genes appears as a common genetic alteration in solid ameloblastoma The current study provides interesting novel information regarding the potential prognostic significance of the allelic loss of the ING gene family loci in ameloblastoma tumorigenesis