MoFap7, a ribosome assembly factor, is required for fungal development and plant colonization of Magnaporthe oryzae
MoFap7, a ribosome assembly factor, is required for fungal development and plant colonization of Magnaporthe oryzae
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MoFap7 是一种核糖体组装因子，是真菌发育和 Magnaporthe oryzae 植物定植所必需的
DOI：
10.1080/21505594.2019.1697123
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发表时间：
2019-01-01
期刊：
影响因子：
5.2
通讯作者：
Lin, Fu-Cheng
中科院分区：
文献类型：
--
作者：
Li, Lin;Zhu, Xue-Ming;Lin, Fu-Cheng
摘要FAP7是一种重要的核糖体组装因子，通过其ATPase活性在酿酒酵母40岁以前核糖体小亚基的合成中起着至关重要的作用。目前，其同系物在丝状真菌中的生物学功能仍不清楚。在水稻稻瘟病菌中发现了一种与ScFap7同源的蛋白MoFap7，它是水稻上一种毁灭性的真菌病原菌，威胁着世界范围内的粮食安全。ΔMofap7突变体在生长发育、分生孢子形态、附着胞形成和侵染等方面存在缺陷，对氧化胁迫敏感。此外，定点突变分析证实，MoFap7中保守的Walker A基序和Walker B基序是米曲霉生物学功能所必需的。我们进一步分析了MoFap7在致病性中的调控机制。MoFap7被发现与MoMst50相互作用，MoMst50是MAPK Pmk1信号通路中的一个调节因子，通过调节MoPmk1的磷酸化参与调节植物的渗透和细胞对细胞的侵袭。此外，MoFap7与GTP酶MoCDc42和Morac1相互作用，控制分生孢子的生长和发生。综上所述，这项研究的结果为MoFap7介导的丝状真菌的发育和发病机制提供了新的见解。
ABSTRACT Fap7, an important ribosome assembly factor, plays a vital role in pre-40S small ribosomal subunit synthesis in Saccharomyces cerevisiae via its ATPase activity. Currently, the biological functions of its homologs in filamentous fungi remain elusive. Here, MoFap7, a homologous protein of ScFap7, was identified in the rice blast fungus Magnaporthe oryzae, which is a devastating fungal pathogen in rice and threatens food security worldwide. ΔMofap7 mutants exhibited defects in growth and development, conidial morphology, appressorium formation and infection, and were sensitive to oxidative stress. In addition, site-directed mutagenesis analysis confirmed that the conserved Walker A motif and Walker B motif in MoFap7 are essential for the biological functions of M. oryzae. We further analyzed the regulation mechanism of MoFap7 in pathogenicity. MoFap7 was found to interact with MoMst50, a regulator functioning in the MAPK Pmk1 signaling pathway, that participates in modulating plant penetration and cell-to-cell invasion by regulating the phosphorylation of MoPmk1. Moreover, MoFap7 interacted with the GTPases MoCdc42 and MoRac1 to control growth and conidiogenesis. Taken together, the results of this study provide novel insights into MoFap7-mediated orchestration of the development and pathogenesis of filamentous fungi.