An Affinity-enhanced DNA Intercalator with Intense ECL Embedded in DNA Hydrogel for Biosensing Applications.
An Affinity-enhanced DNA Intercalator with Intense ECL Embedded in DNA Hydrogel for Biosensing Applications.
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DOI：
10.1021/acs.analchem.0c00152
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发表时间：
2020-07
影响因子：
7.4
通讯作者：
Mei-Ling Zhao;Wei-Jia Zeng;Y. Chai;R. Yuan;Ying Zhuo
中科院分区：
文献类型：
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作者：
Mei-Ling Zhao;Wei-Jia Zeng;Y. Chai;R. Yuan;Ying Zhuo
本研究利用两亲性苝衍生物（PTC-DEDA）作为DNA嵌入剂，构建了一种靶向诱导的DNA水凝胶生物传感平台，用于microRNA let-7a（miRNA let-7a）的灵敏检测。具体地说，具有许多树突状DNA结构的DNA水凝胶是通过靶诱导的非线性杂交链反应在miRNA let-7a的存在下原位产生的，其具有大的负载能力以捕获大量的DNA嵌入剂。然后，由于固有的两亲性结构，带正电荷的PTC-DEDA可以很容易地插入DNA凹槽中，获得强的ECL信号。使用所提出的PTC-DEDA作为DNA嵌入剂和ECL发射体，DNA水凝胶生物传感平台对miRNA let-7a表现出高稳定性和优异的灵敏度，具有理想的线性范围（10 fM至10 nM）和低检测限（1.49 fM）。值得注意的是，这项工作为开发用于生化分析应用的简单高效的ECL平台提供了潜在的替代方案。
Herein, an amphiphilic perylene derivative (denoted as PTC-DEDA) was explored as DNA intercalators endowed with an enhanced affinity and intense electrochemiluminescence (ECL) to construct a target-induced DNA hydrogel biosensing platform for the sensitive detection of microRNA let-7a (miRNA let-7a). Specifically, the DNA hydrogel with numerous dendritic DNA structures was in situ generated via a target-induced nonlinear hybrid chain reaction in the presence of miRNA let-7a, which possessed a large loading capacity to entrap massive DNA intercalators. Then, the PTC-DEDA with positive charges could easily intercalate into the DNA grooves due to the inherent amphipathic structure, achieving a strong ECL signal. Using the proposed PTC-DEDA as both DNA intercalators and ECL emitters, the DNA hydrogel biosensing platform exhibited a high stability and an excellent sensitivity for miRNA let-7a, with a desirable linear range (10 fM to 10 nM) and a low detection limit (1.49 fM). Significantly, the work provided a potential alternative to develop simple and high-efficiency ECL platforms for biochemical analysis applications.