Associations of ACE I/D polymorphism with the levels of ACE, kallikrein, angiotensin II and interleukin-6 in STEMI patients
Associations of ACE I/D polymorphism with the levels of ACE, kallikrein, angiotensin II and interleukin-6 in STEMI patients
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DOI：
10.1038/s41598-019-56263-8
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发表时间：
2019-12-23
影响因子：
4.6
通讯作者：
Liu, Hongyu
中科院分区：
文献类型：
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作者：
Dai, Shuhong;Ding, Mei;Liu, Hongyu
本研究旨在比较中国人群中不同 ACE 插入/缺失 (I/D) 多态性的 ST 段抬高型心肌梗死 (STEMI) 患者血浆血管紧张素 I 转换酶 (ACE)、血管紧张素 II (AngII)、激肽释放酶 (KLK1) 和白细胞介素 6 (IL-6) 的血浆水平。在 199 名 STEMI 患者和 216 名对照受试者中测定了 ACE 基因型。 STEMI 患者根据 ACE 基因型分为三组。进行单聚合酶链反应 (PCR) 来表征 ACE I/D 多态性。通过酶联免疫吸附测定（ELISA）测量ACE、AngII、KLK1和IL-6的血浆水平。我们发现，DD 或 ID 基因型与 STEMI 患者的高 ACE（OR = 4.697；95% CI = 1.927-11.339）、KLK1（3.339；1.383-8.063）和 IL-6 水平（OR = 2.10；1.025-4.327）显着独立相关。然而，无论是单变量还是多变量logistic回归，ACE I/D多态性与AngII血浆水平之间均无统计学意义。此外，我们发现 STEMI 患者血浆 KLK1 水平与 IL-6 水平之间存在显着正相关（r = 0.584，P < 0.001）。研究表明，当 D 等位基因存在时，可检测到高水平的 ACE、KLK1 和 IL-6，但 AngII 血浆水平不受 ACE I/D 多态性的影响。
This study aimed to compare the plasma levels of angiotensin-I converting enzyme (ACE), Angiotensin II (AngII), kallikrein (KLK1) and interleukin-6 (IL-6) in ST segment elevation myocardial infarction (STEMI) patients with different ACE Insertion/deletion (I/D) polymorphisms in a Chinese population. The ACE genotypes were determined in the 199 STEMI patients and 216 control subjects. STEMI patients were divided into three groups based on the ACE genotypes. Single polymerase chain reaction (PCR) was performed to characterize ACE I/D polymorphisms. Plasma levels of ACE, AngII, KLK1 and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). We found that the DD or ID genotype was significantly independently associated with high ACE (OR = 4.697; 95% CI = 1.927-11.339), KLK1 (3.339; 1.383-8.063) and IL-6 levels (OR = 2.10; 1.025-4.327) in STEMI patients. However, there was no statistical significance between the ACE I/D polymorphism and AngII plasma levels whether in univariate or multivariate logistic regression. Additionally, we detected a significantly positive correlation between plasma KLK1 levels and IL-6 levels in STEMI patients (r = 0.584, P < 0.001). The study showed high levels of ACE, KLK1 and IL-6 were detected when the D allele was present, but AngII plasma levels was not influenced by the ACE I/D polymorphism.