Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP
Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP
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DOI：
10.7554/elife.08483
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发表时间：
2015-11-04
期刊：
影响因子：
7.7
通讯作者：
Raff, Jordan W.
中科院分区：
文献类型：
--
作者：
Conduit, Paul T.;Wainman, Alan;Raff, Jordan W.
中心体具有许多重要的功能，包括被中心粒周围物质（PCM）包围的“母亲”和“女儿”中心粒。母亲中心粒招募和组织PCM和模板子中心粒的形成。据报道，几个重要的果蝇PCM组织蛋白从细胞质中被募集到中心粒，作为含有中心粒蛋白Sas-4的大型细胞质“S-CAP”复合物的一部分。在先前的论文（Conduit等人，2014 b）我们表明，这些蛋白质之一Cnn和另一种关键的PCM组织蛋白Spd-2在向外扩散以形成支持有丝分裂PCM组装的支架之前在母亲中心粒周围募集; Cnn和Spd-2的募集依赖于另一种S-CAP蛋白Asl。然而，我们在这里显示，CNN，Spd-2和Asl没有被招募到母亲中心粒作为一个复杂的SAS-4的一部分。因此，PCM招聘苍蝇胚胎似乎并不需要胞质S-CAP复合物。
Centrosomes have many important functions and comprise a 'mother' and 'daughter' centriole surrounded by pericentriolar material (PCM). The mother centriole recruits and organises the PCM and templates the formation of the daughter centriole. It has been reported that several important Drosophila PCM-organising proteins are recruited to centrioles from the cytosol as part of large cytoplasmic 'S-CAP' complexes that contain the centriole protein Sas-4. In a previous paper (Conduit et al., 2014b) we showed that one of these proteins, Cnn, and another key PCM-organising protein, Spd-2, are recruited around the mother centriole before spreading outwards to form a scaffold that supports mitotic PCM assembly; the recruitment of Cnn and Spd-2 is dependent on another S-CAP protein, Asl. We show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4. Thus, PCM recruitment in fly embryos does not appear to require cytosolic S-CAP complexes.