The screening, characterization, and use of ω-laurolactam hydrolase:: A new enzymatic synthesis of 12-aminolauric acid
The screening, characterization, and use of ω-laurolactam hydrolase:: A new enzymatic synthesis of 12-aminolauric acid
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DOI：
10.1271/bbb.80210
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发表时间：
2008-08-01
影响因子：
1.6
通讯作者：
Komeda, Hidenobu
中科院分区：
文献类型：
--
作者：
Asano, Yasuhisa;Fukuta, Yasuhisa;Komeda, Hidenobu
从土壤样品中分离到了几株ω-月桂内酰胺降解菌。这些菌株能够在含有ω-月桂内酰胺作为唯一碳源和氮源的培养基中生长。其中5株（T7、T31、U124、U224和U238）分别鉴定为嗜铜菌T7、嗜酸菌T31、嗜铜菌U124、红球菌U224和鞘氨醇单胞菌U238。从红球菌U224菌株中分离纯化了β-月桂内酰胺水解酶，并对其酶学性质进行了研究。该酶作用于ω-辛内酰胺和ω-月桂内酰胺，但其他内酰胺化合物、酰胺和氨基酸酰胺不能作为底物。克隆了该酶基因，推导的氨基酸序列与节杆菌属K172和假单胞菌属NK 87的6-氨基己酸环二聚体水解酶（EC 3.5.2.12）具有较高的同源性。以红球菌U224的w-月桂内酰胺水解酶为催化剂，酶法合成12-氨基月桂酸。
Several omega-laurolactam degrading microorganisms were isolated from soil samples. These strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. Among them, five strains (T7, T31, U124, U224, and U238) were identified as, Cupriavidus sp. T7, Acidovorax sp. T31, Cupriavidus sp. U124, Rhodococcus sp. U224, and Sphingomonas sp. U238, respectively. The w-laurolactam hydrolyzing enzyme from Rhodococcus sp. U224 was purified to homogeneity, and its enzymatic properties were characterized. The enzyme acts on omega-octalactam and omega-laurolactam, but other lactam compounds, amides and amino acid amides, cannot be substrates. The enzyme, gene was cloned, and the deduced amino acid sequence showed high homology with 6-aminohexanoate-cyclic-dimer hydrolase (EC 3.5.2.12) from Arthrobacter sp. K172 and Pseudomonas sp. NK87. Enzymatic synthesis of 12-aminolauric acid was performed using partially purified w-laurolactam hydrolase from Rhodococcus sp. U224.