A new selective enrichment broth for detecting beta-hemolytic streptococci in throat cultures: quinoline derivate and three percent salt as an additional agent to Pike's inhibitors.
A new selective enrichment broth for detecting beta-hemolytic streptococci in throat cultures: quinoline derivate and three percent salt as an additional agent to Pike's inhibitors.
复制标题
一种用于检测咽喉培养物中 β-溶血性链球菌的新型选择性富集肉汤：喹啉衍生物和 3% 的盐作为派克抑制剂的附加剂。
DOI：
10.1111/j.1348-0421.1972.tb00694.x
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
M. Sato
中科院分区：
文献类型：
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作者：
M. Sato
在日本，派克选择性肉汤[6]已广泛用作链球菌化脓性群的富集培养基。该肉汤含有结晶紫和叠氮化钠作为选择剂。前者对革兰氏阳性菌尤其是葡萄球菌具有杀菌活性，后者对革兰氏阴性菌具有抑菌活性。咽喉中天然存在的细菌，如Stophylococcus，嗜血杆菌和大肠菌群在这种培养基中被很大程度上抑制。派克选择性肉汤支持化脓性链球菌群的生长，但同时允许α-和非溶血性链球菌生长。此后又报道了其他几种选择性药剂。Moustardite等人[4]应用的卡那霉素和制霉菌素，Lowbury等人[2]应用的新霉素、多粘菌素B和夫西地酸，Beerens和Talon-Castel [1]应用的萘啶酸似乎值得一提。然而，这些作者都没有报道在其选择性培养基中或其选择性培养基上成功抑制草绿色组。
In Japan Pike's selective broth [6] has been widely used as an enrichment medium for the pyogenic group of streptococci. This broth contains crystal violet and sodium azide as selective agents. The former exhibits bactericidal activity towards grampositive bacteria, especially staphylococci and the latter is bacteriostatic for gramnegative bacteria. Naturally occurring bacteria in the throat such as Stophylococcus, Hemophilus and coliforms are largely inhibited in this medium. Pike's selective broth supports the growth of the pyogenic group of streptococci, but at the same time permits alpha-and non-hemolytic streptococci to grow. Several other selective agents have since been reported. Kanamycin and mycostatin applied by Moustardiet et al [4], neomycin, polymixin B and fusidic acid by Lowbury et al [2], nalidixic acid by Beerens and Talon-Castel [1] seemed to be worth mentioning. However, none of these authors reported the successful inhibition of the viridans group in or on their selective media.