Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy
Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy
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表面红外光谱无标记实时监测脂肪细胞分化
DOI：
10.1016/j.snb.2012.10.030
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
他
中科院分区：
文献类型：
--
作者：
Yuki Aonuma;Yasuhiko Kondo;Ayumi Hirano-Iwata;他
我们利用具有多重内反射（MIR-IRAS）几何结构的红外吸收光谱研究了细胞向脂肪细胞分化的原位实时监测。在硅棱镜上培养前脂肪细胞3T3-L1细胞，用MIR-IRAS监测其脂肪形成7天。分化后的细胞显示出三酰甘油酯的CO拉伸带，以及蛋白酰胺I和酰胺II带。CO拉伸带出现在光学显微镜下确认脂肪液滴形成之前，脂肪液滴是脂肪细胞的典型形态学标记，这表明MIR-IRAS对于早期脂肪形成的无损监测特别有用。根据CO拉伸和酰胺带的峰值强度，研究了染料木素的抗脂肪作用。因此，MIR-IRAS有潜力评估抗脂肪剂在脂肪形成过程中对脂肪和蛋白质合成的影响。
We have investigated in situ real-time monitoring of cell differentiation to adipocytes by using infrared absorption spectroscopy with the multiple internal reflection (MIR-IRAS) geometry. Preadipocyte 3T3-L1 cells were cultured on a silicon prism and their adipogenesis was monitored with MIR-IRAS for seven days. Differentiated cells showed a CO stretching band of triacylglycerides, together with the protein amide I and amide II bands. The CO stretching band appeared before optical microscopic confirmation of fat droplet formation, a typical morphological marker for adipocytes, demonstrating that MIR-IRAS is particularly useful for non-destructive monitoring of early adipogenesis. An effect of genistein, an antiadipogenic agent, was also investigated based on the peak intensity of the CO stretching and amide bands. Thus, MIR-IRAS has a potential to evaluate antiadipogenic agents in terms of their effects on fat and protein synthesis during adipogenesis.