Cryptic estrogen binding protein complicates analysis of estrogen receptor distribution.
Cryptic estrogen binding protein complicates analysis of estrogen receptor distribution.
复制标题
隐藏的雌激素结合蛋白使雌激素受体分布的分析变得复杂。
DOI：
10.1016/0006-291x(84)90578-3
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发表时间：
1984
影响因子：
3.1
通讯作者：
Muldoon,TG
中科院分区：
文献类型：
--
作者：
Smanik,EJ;Copland,JA;Muldoon,TG
在完整和4周去卵巢的成年动物中研究了大鼠子宫雌激素受体亚型对注射5μg雌二醇的反应。被占据和未被占据的受体亚细胞波动的测定揭示了在仅测量总核和未被占据的胞质受体的标准测定下不可检测的显著差异。两种动物模型都表现出高水平的未被占据的核受体，这是雌激素无法接近的。与完整的动物相反，去势动物的子宫在胞质溶胶中具有高水平的被占据受体，其在雌激素暴露后仍然很高。受体加工发生在去势动物，而不是完整的动物。结果表明，传统的检测方法是复杂的存在和同时测量的非反应性受体物种，定量差异很大的动物模型，并会引起错误的解释雌激素诱导的营业额的受体的模式。
The response of rat uterine estrogen receptor sub-species to injection of 5μg estradiol has been investigated in intact and 4-weeks' ovariectomized adult animals. Determinations of occupied and unoccupied receptor subcellular fluctuations reveal significant differences not detectable under standard assays which measure only total nuclear and unoccupied cytosolic receptors. Both animal models manifest a high level of unoccupied nuclear receptors which are inaccessible to estrogen. In contrast to the intact animal, uteri from castrate animals have a high level of occupied receptors in the cytosol, which remains high following estrogen exposure. Receptor processing occurs in the castrate, but not the intact, animal. The results demonstrate that traditional assays are complicated by the presence and simultaneous measurement of non-responsive receptor species which quantitatively differ widely among animal models and will give rise to an erroneous interpretation of the pattern of estrogen-induced turnover of its receptor.