PURIFICATION TO HOMOGENEITY OF B-CELL STIMULATING FACTOR - A MOLECULE THAT STIMULATES PROLIFERATION OF MULTIPLE LYMPHOKINE-DEPENDENT CELL-LINES
PURIFICATION TO HOMOGENEITY OF B-CELL STIMULATING FACTOR - A MOLECULE THAT STIMULATES PROLIFERATION OF MULTIPLE LYMPHOKINE-DEPENDENT CELL-LINES
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DOI：
10.1084/jem.163.6.1405
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发表时间：
1986-06-01
影响因子：
15.3
通讯作者：
GILLIS, S
中科院分区：
文献类型：
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作者：
GRABSTEIN, K;EISENMAN, J;GILLIS, S
从小鼠胸腺瘤细胞系的培养上清液中纯化出均一的小鼠B细胞刺激因子1（BSF - 1）。鉴定出一种分子量为18.4 kD且具有独特N端氨基酸序列的蛋白质。BSF - 1的比活性至少为3.28×10⁸单位/毫克。除了具有刺激B细胞的活性外，BSF - 1还能刺激几种白细胞介素 - 2（IL - 2）和白细胞介素 - 3（IL - 3）依赖的细胞系增殖。我们得出结论：BSF - 1既是一种生长因子也是一种分化因子。最后，这些结果还表明BSF - 1对除B淋巴细胞以外的细胞谱系具有其他生物学特性。
Murine B cell stimulating factor 1 (BSF-1) was purified to homgeneity from supernatants of a stimulated thymoma cell line. A protein of 18.4 kD with a unique N-terminal amino acid sequence was identified. BSF-1 had a sp act of at least 3.28 .times. 108 U/mg. In addition to its B cell-stimulatory activity, BSF-1 also stimulated the proliferation of several IL-2- and IL-3-dependent cell lines. We conclude that BSF-1 is both a growth factor and a differentiation factor. Finally, these results also suggest additional biologic properties of BSF-1 on lineages besides B lymphocytes.