Anti-inflammatory activity of Korean thistle Cirsium maackii and its major flavonoid, luteolin 5-O-glucoside
Anti-inflammatory activity of Korean thistle Cirsium maackii and its major flavonoid, luteolin 5-O-glucoside
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DOI：
10.1016/j.fct.2012.04.011
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发表时间：
2012-06-01
影响因子：
4.3
通讯作者：
Choi, Jae Sue
中科院分区：
文献类型：
--
作者：
Jung, Hyun Ah;Jin, Seong Eun;Choi, Jae Sue
评估了整个大蓟（菊科）植物及其主要类黄酮木犀草素 5-O-葡萄糖苷的抗炎活性，评估其抑制脂多糖 (LPS) 诱导的一氧化氮 (NO) 产生、诱导型一氧化氮合酶 (iNOS)、环氧合酶-2 (COX-2) 蛋白表达和叔丁基过氧化氢 (t-BHP) 诱导的活性氧的能力(ROS) 在 RAW 264.7 鼠巨噬细胞中产生。 C maackii 的甲醇提取物显示出很强的抗炎活性，因此可以用几种溶剂进行分级。乙酸乙酯可溶部分表现出最高的抗炎活性潜力，进一步产生主要的类黄酮，木犀草素 5-O-葡萄糖苷。我们发现，在 RAW 264.7 细胞中，无毒浓度的木犀草素 5-0-葡萄糖苷以剂量依赖性方式抑制 LPS 诱导的 NO 产生和 t-BHP 诱导的 ROS 产生。它还抑制 LPS 刺激的巨噬细胞中 iNOS 和 COX-2 的表达。此外，通过 HPLC 分析，C. maackii 的甲醇提取物抑制 NO 和 ROS 的功效归因于其类黄酮含量。这些结果表明，C. maackii全草及其黄酮类化合物通过抑制ROS的产生来抑制iNOS和COX-2的表达，因此可以被认为是治疗各种炎症和氧化应激相关疾病的有用的治疗和预防方法。 (C) 2012 Elsevier Ltd. 保留所有权利。
The anti-inflammatory activity of whole Cirsium maackii (family Compositae) plants and of its major flavonoid, luteolin 5-O-glucoside, was evaluated for their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) protein expression, and tert-butylhydroperoxide (t-BHP)-induced reactive oxygen species (ROS) generation in RAW 264.7 murine macrophage cells. The methanolic extract of C maackii showed strong anti-inflammatory activity, and was thus fractionated with several solvents. The ethyl acetate-soluble fraction, exhibiting the highest anti-inflammatory activity potential, was further to yield a major flavonoid, luteolin 5-O-glucoside. We found that luteolin 5-0-glucoside, at a non-toxic concentration, inhibited LPS-induced NO production and t-BHP-induced ROS generation in a dose-dependent manner in RAW 264.7 cells. It also suppressed the expression of iNOS and COX-2 in LPS-stimulated macrophages. Furthermore, the efficacies of the methanolic extract of C. maackii in inhibiting both NO and ROS were attributed to its flavonoid content by HPLC analysis. These results indicated that C. maackii whole plants and its flavonoids inhibit the expression of iNOS and COX-2 in through the inhibition of ROS generation, and therefore can be considered as a useful therapeutic and preventive approach for the treatment of various inflammatory and oxidative stress-related diseases. (C) 2012 Elsevier Ltd. All rights reserved.