VIBRIO-ALGINOLYTICUS, A TETRODOTOXIN-PRODUCING BACTERIUM, IN THE INTESTINES OF THE FISH FUGU-VERMICULARIS-VERMICULARIS
VIBRIO-ALGINOLYTICUS, A TETRODOTOXIN-PRODUCING BACTERIUM, IN THE INTESTINES OF THE FISH FUGU-VERMICULARIS-VERMICULARIS
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DOI：
10.1007/bf00431409
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发表时间：
1987-01-01
期刊：
影响因子：
2.4
通讯作者：
HASHIMOTO, K
中科院分区：
文献类型：
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作者：
NOGUCHI, T;HWANG, DF;HASHIMOTO, K
为阐明河豚毒素对动物的毒害机制，于1985年对河豚肠道内容物进行了细菌群检测。33个菌株中有26个属于弧菌属。根据生物学和生化特性，将这些细菌分为I到VII组。高效液相色谱、气相色谱-质谱联用和小鼠生物毒性试验表明，在由植酸体蛋白内酯(BBL)和氯化钠组成的培养基中，第I组细菌产生河豚毒素和脱水河豚毒素。其他一些群体也在一定程度上产生了这种毒素和/或相关物质。I组菌株均鉴定为溶藻弧菌。根据应用的所有仪器分析，四个菌株中有两个产生了可检测到的河豚毒素和/或脱水河豚毒素。我们的发现表明，一些溶藻弧菌与河豚的中毒密切相关，可能也与其他物种的中毒密切相关。
To clarify the mechanism of toxification in animals contaminated with tetrodotoxin, the intestinal contents of the puffer Fugu vermicularis vermicularis were examined for bacterial flora in 1985. Twenty-six out of 33 strains belonged to the genus Vibrio. These bacteria were classified into Groups I to VII, based on biological and biochemical characters. High performance liquid chromatography and gas chromatograpy-mass spectrometry, together with mouse bioassay for toxicity, clearly demonstrated that Group I produced tetrodotoxin and anhydrotetrodotoxin under cultivation with a medium composed of Phytone peptone (BBL) and NaCl. Some other groups also produced this toxin and/or related substances to some extent. Strains of Group I were all identified as Vibrio alginolyticus. Two strains among four produced a detectable amount of tetrodotoxin and/or anhydrotetrodotoxin, as measured by all instrumental analyses applied. Our findings suggest that some strains of V. alginolyticus are closely related to the toxification of the puffer, and probably of other species.