CORRELATIONS BETWEEN IMMEDIATE-EARLY GENE INDUCTION AND THE PERSISTENCE OF LONG-TERM POTENTIATION
CORRELATIONS BETWEEN IMMEDIATE-EARLY GENE INDUCTION AND THE PERSISTENCE OF LONG-TERM POTENTIATION
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DOI：
10.1016/0306-4522(93)90369-q
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发表时间：
1993-10-01
期刊：
影响因子：
3.3
通讯作者：
DRAGUNOW, M
中科院分区：
文献类型：
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作者：
ABRAHAM, WC;MASON, SE;DRAGUNOW, M
在系统地控制所传递的刺激序列的数量后，检查了清醒大鼠齿状回的长期增强的持续时间。这与给予相同刺激方案的不同动物组中立即早期基因的诱导有关。经过 10 组刺激后，长时程增强以长达几天的时间常数衰减（长时程增强 2），这与 zif/268 信使 RNA 和蛋白质水平的增加相关。增加刺激序列的数量导致引发时间常数为几周的长期增强的可能性更大（长期增强3），以及增加 zif/268、c-Jun、Jun-B 的诱导。 Jun-D 和 Fos 相关蛋白。当连续五天重复交付 10 个列车时，只有 zif/268 蛋白水平显示出相关变化。这些数据支持了以下假设：长期增强 3 涉及长期增强 2 之外的机制。一种可能的机制是改变基因表达，由立即早期基因转录因子（例如 zif/268 以及可能的 Fos 和 Jun 家族成员的同二聚体或异二聚体）启动，然后促进基因表达的改变。 3. 长时程增强的稳定或维持。
The duration of long-term potentiation in the dentate gyrus of awake rats was examined following systematic manipulation of the number of stimulus trains delivered. This was correlated with the induction of immediate early genes in separate groups of animals given identical stimulus regimes. Following 10 trains of stimulation, long-term potentiation decayed with a time constant of up to several days (long-term potentiation 2), and this correlated with the appearance of an increase in the messenger RNA and protein levels of zif/268. Increasing the number of stimulus trains resulted in a greater probability of eliciting long-term potentiation with a time constant of several weeks (long-term potentiation 3), as well as increasing the induction of zif/268, c-Jun, Jun-B. Jun-D and Fos-related proteins. When 10 trains were delivered repeatedly on up to five consecutive days, only the zif/268 protein levels showed associated changes.These data provide support for the hypothesis that long-term potentiation 3 involves mechanisms additional to those for long-term potentiation 2. One possible mechanism is altered gene expression, initiated by immediate early gene transcription factors such as zif/268 and possibly homo- or heterodimers of Fos and Jun family members, that then contributes to the stabilization or maintenance of long-term potentiation 3.