Detecting structural changes in early experimental osteoarthritis of tibial cartilage by microscopic magnetic resonance imaging and polarised light microscopy
Detecting structural changes in early experimental osteoarthritis of tibial cartilage by microscopic magnetic resonance imaging and polarised light microscopy
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DOI：
10.1136/ard.2003.011783
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发表时间：
2004-06-01
影响因子：
27.4
通讯作者：
Matyas, JR
中科院分区：
文献类型：
--
作者：
Alhadlaq, HA;Xia, Y;Matyas, JR
目的：应用显微磁共振成像(MuMRI)和偏光显微镜(PLM)观察早期骨关节炎(OA)犬模型关节软骨中胶原纤维网络的变化。方法：对3对前交叉韧带切断犬胫骨内侧标本12周后进行MuMRI和PLM研究。对每个标本进行以下实验：(A)四个方向的T-2松弛的二维MuMRI图像；(B)切线杨氏模数；(C)光学延迟和纤维角的二维PLM图像。以13.7-23.1 mm的多重MRI分辨率观察病变引起的组织变化。结果：在膝关节骨性关节炎的胫骨软骨的T-2加权图像上可以看到几个明显的变化。对于至少部分被半月板覆盖的标本，MuMRI的显著变化包括最大T-2深度明显移位(21-36%)，浅层厚度减少(37%-38%)，软骨总厚度增加(15%-27%)。这些多层磁共振成像的改变在胫骨表面的地形上有所不同，因为它们在胫骨内侧完全暴露的位置并不明显。结论：MuMRI和PLM均能定量检测早期骨性关节炎时胶原纤维构筑的变化，并能解决犬胫骨软骨微结构的地形性变化。
Objectives: To detect changes in the collagen fibril network in articular cartilage in a canine experimental model of early osteoarthritis (OA) using microscopic magnetic resonance imaging (muMRI) and polarised light microscopy (PLM).Methods: Eighteen specimens from three pairs of the medial tibia of an anterior cruciate ligament transection canine model were subjected to muMRI and PLM study 12 weeks after surgery. For each specimen, the following experiments were carried out: ( a) two dimensional muMRI images of T-2 relaxation at four orientations; (b) the tangent Young's modulus; and ( c) two dimensional PLM images of optical retardance and fibril angle. Disease induced changes in tissue were examined across the depth of the cartilage at a muMRI resolution of 13.7 - 23.1 mum.Results: Several distinct changes from T-2 weighted images of cartilage in OA tibia were seen. For the specimens that were covered at least in part by the meniscus, the significant changes in muMRI included a clear shift in the depth of maximum T-2 ( 21 - 36%), a decrease in the superficial zone thickness (37 - 38%), and an increase in cartilage total thickness (15 - 27%). These muMRI changes varied topographically in the tibia surface because they were not significant in completely exposed locations in medial tibia. The muMRI results were confirmed by the PLM measurements and correlated well with the mechanical measurements.Conclusion: Both muMRI and PLM can detect quantitatively changes in collagen fibre architecture in early OA and resolve topographical variations in cartilage microstructure of canine tibia.