Ectomycorrhizal Association Enhances Al Tolerance by Inducing Citrate Secretion in Pinus densiflora
Ectomycorrhizal Association Enhances Al Tolerance by Inducing Citrate Secretion in Pinus densiflora
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DOI：
10.1111/j.1747-0765.2005.tb00045.x
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发表时间：
2005-06
影响因子：
2
通讯作者：
Ko Tahara;M. Norisada;T. Tange;Hisayoshi Yagi;K. Kojima
中科院分区：
文献类型：
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作者：
Ko Tahara;M. Norisada;T. Tange;Hisayoshi Yagi;K. Kojima
研究外生菌根对赤松耐铝性的影响。& Zucc.，幼苗有或没有外生菌根协会与真菌Pisolithus tinctorius（E.A.）Coker & Couch暴露于1 mM Al。与P. tinctorius联合缓解了Al诱导的菌根幼苗根伸长和生物量生长的抑制。苹果酸和柠檬酸，这两个低分子量的有机酸，可以解毒铝形成稳定的络合物，分泌进行了研究，在P. tinctorius菌丝体和松树根与和没有P. tinctorius协会。铝能促进有色青霉菌丝体的柠檬酸分泌，也能促进外生菌根幼苗根系的柠檬酸分泌，但在非菌根幼苗中没有检测到。这些结果表明，从外生菌根幼苗的根分泌的柠檬酸盐是在P.tinctorius的菌丝中产生的。柠檬酸的分泌可能在提高寄主幼苗的耐铝性中起作用。
To investigate the effects of ectomycorrhizal association on the aluminum (Al) tolerance of Pinus densiflora Sieb. & Zucc., seedlings with or without ectomycorrhizal association with the fungus Pisolithus tinctorius (Pers.) Coker & Couch were exposed to 1 mM Al. Association with P. tinctorius alleviated Al-induced inhibition of root elongation and biomass growth in the mycorrhizal seedlings. Secretion of malate and citrate, both low-molecular-weight organic acids that could detoxify Al by the formation of stable complexes, was investigated in P. tinctorius mycelia and in pine roots with and without P. tinctorius association. Citrate secretion from the P. tinctorius mycelia in vitro was stimulated by Al. Citrate secretion from the roots of the ectomycorrhizal seedlings was also stimulated by Al, but was not detected in the non-mycorrhizal seedlings. These results suggest that citrate secreted from the roots of the ectomycorrhizal seedlings was produced in the hyphae of P. tinctorius. Citrate secretion may play a role in enhancing the Al tolerance of host seedlings.