Thyroid hormone-regulated expression of nuclear lamins correlates with dediffe-rentiation of intestinal epithelial cells during Xenopus laevis metamorphosis
Thyroid hormone-regulated expression of nuclear lamins correlates with dediffe-rentiation of intestinal epithelial cells during Xenopus laevis metamorphosis
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甲状腺激素调节的核纤层蛋白表达与非洲爪蟾变态过程中肠上皮细胞的去分化相关
DOI：
10.1007/s00427-011-0371-7
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ishizuya-Oka A
中科院分区：
文献类型：
--
作者：
Hasebe T.;Kajita M.;Iwabuchi M.;Ohsumi K.;Ishizuya-Oka A
在由甲状腺激素（TH）引发的变态过程中，成年爪蟾的上皮发育并取代经历凋亡的幼虫上皮。我们以前已经证明成体上皮的祖细胞/干细胞起源于一些分化的幼虫上皮细胞。为了研究幼虫上皮细胞去分化为成体祖细胞/干细胞的分子机制，我们在这里重点研究了核层蛋白A （LA）和层蛋白LIII (LIII)，它们的表达通常与细胞分化状态相关。我们分析了LA和LIII在x期间的时空表达。通过反转录PCR、免疫印迹和免疫组织化学方法研究小鼠肠道重塑。在自然变态开始时，当成年上皮祖细胞出现小胰岛时，LA的表达下调，而LIII的表达仅在胰岛上调。然后，随着成体祖细胞的分化，LA的表达上调，LIII的表达下调。随着多个肠褶皱的形成，LIII阳性的成人上皮细胞仅局限于褶皱的凹槽。此外，我们已经证明TH上调或下调这些层蛋白在预变质肠中的表达，就像在自然变态过程中一样。这些结果表明，th调节的LA和LIII的表达与上皮细胞的去分化密切相关。laevis肠，提示在两栖动物的变态过程中，纤层蛋白参与了去分化过程。
In theXenopus laevisintestine during metamorphosis, which is triggered by thyroid hormone (TH), the adult epithelium develops and replaces the larval one undergoing apoptosis. We have previously shown that progenitor/stem cells of the adult epithelium originate from some differentiated larval epithelial cells. To investigate molecular mechanisms underlying larval epithelial dedifferentiation into the adult progenitor/stem cells, we here focused on nuclear lamin A (LA) and lamin LIII (LIII), whose expression is generally known to be correlated with the state of cell differentiation. We analyzed the spatiotemporal expression of LA and LIII duringX. laevisintestinal remodeling by reverse transcription PCR, Western blotting, and immunohistochemistry. At the onset of natural metamorphosis, when the adult epithelial progenitor cells appear as small islets, the expression of LA is down-regulated, but that of LIII is up-regulated only in the islets. Then, as the adult progenitor cells differentiate, the expression of LA is up-regulated, whereas that of LIII is down-regulated in the adult cells. As multiple intestinal folds form, adult epithelial cells positive for LIII become restricted only to the troughs of the folds. In addition, we have shown that TH up- or down-regulates the expression of these lamins in the premetamorphic intestine as during natural metamorphosis. These results indicate that TH-regulated expression of LA and LIII closely correlates with dedifferentiation of the epithelial cells in theX. laevisintestine, suggesting the involvement of the lamins in the process of dedifferentiation during amphibian metamorphosis.