General approach for introduction of various chemical labels in specific RNA locations based on insertion of amino linkers.
General approach for introduction of various chemical labels in specific RNA locations based on insertion of amino linkers.
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基于氨基接头的插入，在特定RNA位置引入各种化学标签的一般方法。
DOI：
10.3390/molecules181214455
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发表时间：
2013-11-25
期刊：
影响因子：
--
通讯作者：
Karpova G
中科院分区：
文献类型：
--
作者：
Graifer D;Karpova G
在设计的RNA位点引入报告基团是一种被广泛接受的方法，以获得关于RNA与其他生物聚合物在不同细胞过程中形成的络合物中的分子环境的信息。在设计的位置获得携带不同报告基团的RNA的一般方法是基于特定位置插入含有主要脂肪胺功能基团(氨基连接体)的基团，然后用适当的化学物质选择性地衍生它们。本文对不同RNA中氨基连接物的位点特异性引入方法进行了简要的综述。这些方法包括：(I)在化学合成过程中将携带氨基连接物或可被其取代的功能的核苷掺入寡核苷酸中；(Ii)通过连接短合成片段来组装含有氨基连接物的RNA；(Iii)使用T7 RNA聚合酶合成氨基连接物修饰的RNA；(Iv)将氨基连接物插入到特定类型的官能团上的未修饰RNA中，例如鸟苷残基的5‘-磷酸和N7；以及(V)利用基于核酸序列特异性修饰的方法将氨基连接物引入长的高结构RNA中。介绍了用于含氨基连接的RNA衍生化的特殊报告基团，以及所获得的RNA衍生物的类型及其应用领域。
Introduction of reporter groups at designed RNA sites is a widely accepted approach to gain information about the molecular environment of RNAs in their complexes with other biopolymers formed during various cellular processes. A general approach to obtain RNAs bearing diverse reporter groups at designed locations is based on site-specific insertion of groups containing primary aliphatic amine functions (amino linkers) with their subsequent selective derivatization by appropriate chemicals. This article is a brief review on methods for site-specific introduction of amino linkers in different RNAs. These methods comprise: (i) incorporation of a nucleoside carrying an amino-linker or a function that can be substituted with it into oligoribonucleotides in the course of their chemical synthesis; (ii) assembly of amino linker-containing RNAs from short synthetic fragments via their ligation; (iii) synthesis of amino linker-modified RNAs using T7 RNA polymerase; (iv) insertion of amino linkers into unmodified RNAs at functional groups of a certain type such as the 5'-phosphates and N7 of guanosine residues and (v) introduction of an amino linker into long highly structured RNAs exploiting an approach based on sequence-specific modification of nucleic acids. Particular reporter groups used for derivatization of amino linker-containing RNAs together with types of RNA derivatives obtained and fields of their application are presented.
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影响因子：
14.9
作者：
Laletina E;Graifer D;Malygin A;Ivanov A;Shatsky I;Karpova G
通讯作者：
Karpova G
DOI：
10.1016/s0167-4781(03)00072-1
发表时间：
2003-05-13
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Demeshkina, N;Laletina, E;Karpova, G
通讯作者：
Karpova, G
影响因子：
--
作者：
Kojima, Naoshi;Komatsu, Yasuo
通讯作者：
Komatsu, Yasuo
DOI：
10.1073/pnas.1219305110
发表时间：
2013-04-23
影响因子：
11.1
作者：
Crawford, Daniel J.;Hoskins, Aaron A.;Moore, Melissa J.
通讯作者：
Moore, Melissa J.
DOI：
10.1261/rna.036871.112
发表时间：
2013-08
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Kossinova O;Malygin A;Krol A;Karpova G
通讯作者：
Karpova G