Physical and immunochemical properties of low-buoyant-density proteoglycans from avian cartilage.
Physical and immunochemical properties of low-buoyant-density proteoglycans from avian cartilage.
复制标题
禽类软骨低浮力密度蛋白多糖的物理和免疫化学特性。
DOI：
10.1016/0003-9861(81)90361-1
复制
发表时间：
1981
影响因子：
3.9
通讯作者：
Goetinck,PF
中科院分区：
文献类型：
--
作者：
McKeown-Longo,PJ;Sparks,KJ;Goetinck,PF
来自禽类剑状软骨的蛋白多糖连接组分 (A1D5) 含有单连接蛋白和低浮力密度蛋白多糖。针对该组分制备的抗血清（抗 A1D5 血清）特异性结合来自 14 天胚胎鸡胸骨合成的聚集体 (A1)、单体 (A1D1) 和连接 (A1D5) 组分的 [35S]硫酸盐标记的蛋白聚糖。当使用来自单体 (A1D1) 或连接 (A1D5) 级分的 [35S] 硫酸盐标记的蛋白多糖作为抗原时，抗 A1D5 血清以及针对纯化单体制剂的抗血清（抗 A1D11400 Vo 血清）会结合。然而，单体部分中蛋白聚糖的还原和烷基化确实揭示了两种抗血清结合的差异。尽管抗原 (35S-A1D1) 的还原和烷基化会抑制两种抗血清的结合，但抗 A1D5 血清的结合更容易受到抗原改变的影响。两种抗血清的差异也可以在使用标记聚集体作为抗原的测定中检测到。当在抗体稀释曲线中进行比较时，抗A1D5血清不能维持与抗A1D1-1400 Vo血清相同的抗原结合水平。此外，抗A1D1-1400 Vo血清可以在含有4m盐酸胍的蔗糖密度梯度沉淀过程中稳定聚集体，而抗A1D5血清则不能。这些结果表明，在禽类软骨的连接 (A1D5) 级分中发现的低浮力密度蛋白多糖含有在蛋白多糖单体上发现的一些抗原决定簇。然而，至少有一个单体决定簇不存在于A1D5级分中。
A proteoglycan link fraction (A1D5) from avian xyphoid cartilage contains one link protein and low-buoyant-density proteoglycans. An antiserum made against this fraction (anti-A1D5serum) specifically binds [35S]sulfate-labeled proteoglycans from aggregate (A1), monomer (A1D1), and link (A1D5) fractions synthesized by 14-day embryonic chick sterna. When [35S]sulfate-labeled proteoglycans from either monomer (A1D1) or link (A1D5) fractions are used as the antigen, anti-A1D5serum binds as well as an antiserum against a purified monomer preparation (anti-A1D11400 Vo serum). However, reduction and alkylation of the proteoglycans from the monomer fraction does reveal differences in the binding of the two antisera. Whereas reduction and alkylation of the antigen (35S-A1D1) inhibit binding by both antisera, binding of the anti-A1D5serum is more affected by antigen alteration. Differences in the two antisera can also be detected in assays using labeled aggregate as antigen. Anti-A1D5serum cannot maintain the same levels of antigen binding as the anti-A1D1-1400 Vo serum when compared in an antibody dilution curve. In addition, anti-A1D1-1400 Vo serum can stabilize aggregate during sedimentation in a sucrose density gradient containing 4mguanidine hydrochloride whereas anti-A1D5serum cannot. These results suggest that the low-buoyant-density proteoglycans found in the link (A1D5) fraction from avian cartilage contain some of the antigenic determinants found on the proteoglycan monomer. However, there is at least one monomer determinant which is not present in the A1D5fraction.