Dissecting inhibitory effect of boric acid on virulence and patulin production of Penicillium expansum
Dissecting inhibitory effect of boric acid on virulence and patulin production of Penicillium expansum
复制标题
硼酸对扩展青霉毒力和展青霉素产生的抑制作用的剖析
DOI：
10.1016/j.postharvbio.2016.03.003
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发表时间：
2016-07-01
影响因子：
7
通讯作者：
Zhou, Ting
中科院分区：
文献类型：
--
作者：
Lai, Tongfei;Wang, Ying;Zhou, Ting
扩展青霉菌是蓝霉病的致病菌，是仁果采后最具破坏性的病原体之一。它也被认为是霉菌毒素棒曲霉素的主要来源，可导致严重的健康问题。在本研究中，评估了外源硼酸（BA）对 P. expansum 毒力和展青霉素产生的影响。结果表明，0.3%的BA显着抑制P. expansum离体发育，降低对苹果果实的毒力。此外，BA还抑制碳水化合物、脂质和蛋白质的代谢，减少展青霉素的产生。进一步分析表明，BA处理下涉及棒曲霉素生物合成的15个基因中的大多数基因的表达均下调。此外，通过基于 iTRAQ（用于相对和绝对定量的同量异位标签）定量蛋白质组分析获得了 P. expansum 孢子响应 0.3% BA 的蛋白质组变化的全局视图。共鉴定出315个差异表达蛋白（DEP）（87个上调，228个下调），这些DEP主要涉及碳水化合物和蛋白质代谢途径。其中，脓毒蛋白和与核糖体成分或核糖体生物合成相关的蛋白质因其显着上调而受到关注。我们的结果为理解 BA 调节 P. expansum 生长及其毒力的机制提供了新的信息。 (C) 2016 Elsevier B.V. 保留所有权利。
Pencillium expansum, the causal agent of blue mould rot, is one of the most destructive postharvest pathogens of pome fruit. It is also considered as the main source of the mycotoxin patulin which can lead to serious health problems. In the present study, the effects of exogenous boric acid (BA) on virulence and patulin production of P. expansum were evaluated. The results showed that 0.3% BA significantly inhibited the development of P. expansum in vitro and resulted in lower virulence to apple fruit. Moreover, BA suppressed the metabolism of carbohydrate, lipid and protein, reduce patulin production in P. expansum. Further analysis revealed that the expressions of the majority of the 15 genes involved in patulin biosynthesis were down-regulated under BA treatment. In addition, a global view of proteome alteration of P. expansum spores in response to 0.3% BA was acquired by an iTRAQ-based (Isobaric tags for relative and absolute quantitation) quantitative proteomic analysis. A total of 315 differentially expressed proteins (DEPs) (87 up-regulated and 228 down-regulated) were identified, and these DEPs were mainly involved in carbohydrate and protein metabolic pathways. Among them, septins and proteins related to ribosome component or ribosome biosynthesis were noticed for their significant up-regulation. Our results provided novel information for understanding the mechanism, by which BA regulated the growth of P. expansum and its virulence. (C) 2016 Elsevier B.V. All rights reserved.