Extraction and purification of crocin from saffron stigmas employing a simple and efficient crystallization method.
Extraction and purification of crocin from saffron stigmas employing a simple and efficient crystallization method.
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DOI：
10.3923/pjbs.2010.691.698
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发表时间：
2010-07-15
期刊：
影响因子：
--
通讯作者：
Seifi, M
中科院分区：
文献类型：
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作者：
Hadizadeh, F;Mohajeri, S A;Seifi, M
采用结晶法从藏红花柱头中提取藏红花总苷。优选出80%乙醇为最佳提取溶剂。在不同的温度下进行了一步和两步结晶过程。以80%乙醇为结晶介质。从第一次结晶获得的藏红花素晶体具有低纯度，因此进行第二次结晶。在-5 ℃下的第二次结晶中得到较高纯度的晶体。用紫外可见分光光度法和高效液相色谱法研究了藏红花素晶体的纯度，并与Fluka产品和藏红花柱头甲醇提取物进行了比较。结果表明，其纯度极高，约为Fluka产品的13倍。尽管我们的预期，Fluka产品不是纯的α-藏红花素样品;除了一种未知的杂质外，在其色谱图中还观察到五种其他类型的藏红花素。本研究中结晶得到的西红花总甙纯度大于97%。
In this study, total crocin was extracted from saffron stigmas using crystallization method. Ethanol 80% was selected as the best extraction solvent. Crystallization process was carried out in one and two steps at different temperatures. Ethanol 80% was used as crystallization medium. Crocin crystals obtained from the first crystallization had low purity and thus were subjected to the second crystallization. The higher purity crystals were yielded in the second crystallization at -5 degrees C. The purity of crocin crystals was studied using UV-visible spectrophotometery and HPLC in comparison with Fluka product and methanolic extract of saffron stigmas. The results indicated that its purity was extremely higher, about 13 times, more than Fluka product. In spite of our expectation, the Fluka product was not a pure alpha-crocin sample; five other types of crocins in addition to an unknown impurity were seen in its chromatogram. The purity of crystallized total crocin in this work was more than 97%.