Proteomic Analysis of Vesicle-Producing Pseudomonas aeruginosa PAO1 Exposed to X-Ray Irradiation.
Proteomic Analysis of Vesicle-Producing Pseudomonas aeruginosa PAO1 Exposed to X-Ray Irradiation.
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X 射线照射下产生囊泡的铜绿假单胞菌 PAO1 的蛋白质组学分析
DOI：
10.3389/fmicb.2020.558233
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang ZL
中科院分区：
文献类型：
--
作者：
Zhang L;Zhao SQ;Zhang J;Sun Y;Xie YL;Liu YB;Ma CC;Jiang BG;Liao XY;Li WF;Cheng XJ;Wang ZL
电离辐射通过破坏核酸而不破坏蛋白质结构来杀死病原体。然而，病原体如何应对辐射胁迫尚未完全阐明。在这里，我们观察到铜绿假单胞菌PAO1可以释放核酸到细胞外环境下的X射线照射。利用扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察X射线照射诱导铜绿假单胞菌PAO 1外膜囊泡（OMV）的形成。根据纳米颗粒跟踪分析（NTA），辐照的PAO 1的OMV的尺寸分布与未辐照的PAO 1的OMV的尺寸分布相似。绿脓菌素相关蛋白参与X射线照射条件下铜绿假单胞菌PAO1中OMV的产生，并且这受到关键SOS基因recA的调控。辐照后PAO1 Δlys突变体的OMV产量显著受损，表明Lys内溶素与铜绿假单胞菌PAO1在辐照胁迫下的OMV产量相关。同时，在缺少pqsR、lasR或rhlR基因的PAO 1和亲本菌株之间没有观察到OMV产量的显著差异，表明辐射诱导的铜绿假单胞菌的OMV生物合成不依赖于假单胞菌喹诺酮信号（PQS）。
Ionizing irradiation kills pathogens by destroying nucleic acids without protein structure destruction. However, how pathogens respond to irradiation stress has not yet been fully elucidated. Here, we observed that Pseudomonas aeruginosa PAO1 could release nucleic acids into the extracellular environment under X-ray irradiation. Using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), X-ray irradiation was observed to induce outer membrane vesicle (OMV) formation in P. aeruginosa PAO1. The size distribution of the OMVs of the irradiated PAO1 was similar to that of the OMVs of the non-irradiated PAO1 according to nanoparticle tracking analysis (NTA). The pyocin-related proteins are involved in OMV production in P. aeruginosa PAO1 under X-ray irradiation conditions, and that this is regulated by the key SOS gene recA. The OMV production was significantly impaired in the irradiated PAO1 Δlys mutant, suggesting that Lys endolysin is associated with OMV production in P. aeruginosa PAO1 upon irradiation stress. Meanwhile, no significant difference in OMV production was observed between PAO1 lacking the pqsR, lasR, or rhlR genes and the parent strain, demonstrating that the irradiation-induced OMV biosynthesis of P. aeruginosa was independent of the Pseudomonas quinolone signal (PQS).
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影响因子：
14.8
作者：
Hmelo LR;Borlee BR;Almblad H;Love ME;Randall TE;Tseng BS;Lin C;Irie Y;Storek KM;Yang JJ;Siehnel RJ;Howell PL;Singh PK;Tolker-Nielsen T;Parsek MR;Schweizer HP;Harrison JJ
通讯作者：
Harrison JJ
影响因子：
6.4
作者：
Florez C;Raab JE;Cooke AC;Schertzer JW
通讯作者：
Schertzer JW
影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
3.6
作者：
Hamilton, HL;Domínguez, NM;Dillard, JP
通讯作者：
Dillard, JP
影响因子：
6.7
作者：
Gamalier, Juliana P.;Silva, Thiago P.;Melo, Rossana C. N.
通讯作者：
Melo, Rossana C. N.