In vivo magnetic resonance imaging and semiautomated image analysis extend the brain phenotype for cdf/cdf mice
In vivo magnetic resonance imaging and semiautomated image analysis extend the brain phenotype for cdf/cdf mice
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DOI：
10.1523/jneurosci.5438-05.2006
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发表时间：
2006-04-26
影响因子：
5.3
通讯作者：
Henkelman, RM
中科院分区：
文献类型：
--
作者：
Bock, NA;Kovacevic, N;Henkelman, RM
人类临床研究中的磁共振成像和计算机图像分析可有效识别疾病人群中的异常神经解剖结构。随着更多神经系统疾病的小鼠模型被发现，这种方法可能被证明对转化研究有用。在这里，我们通过对具有小脑缺叶（cdf）突变的小鼠进行表型分析，证明了类似策略在小鼠神经科学研究中的有效性。使用体内多小鼠磁共振成像来增加通量，我们对cdf突变、杂合和野生型小鼠组进行成像，并对15个个体大脑中的结构进行基于图谱的分割。然后，我们对结构进行计算机自动体积测量。我们在cdf突变体中发现小脑体积减小，这是预期的，但我们也在下丘和嗅球中发现了一种新的表型。随后的局部组织学检查显示嗅球细胞结构异常。这证明了利用解剖磁共振成像和半自动图像分析检测突变小鼠的异常神经结构。
Magnetic resonance imaging and computer image analysis in human clinical studies effectively identify abnormal neuroanatomy in disease populations. As more mouse models of neurological disorders are discovered, such an approach may prove useful for translational studies. Here, we demonstrate the effectiveness of a similar strategy for mouse neuroscience studies by phenotyping mice with the cerebellar deficient folia (cdf) mutation. Using in vivo multiple-mouse magnetic resonance imaging for increased throughput, we imaged groups of cdf mutant, heterozygous, and wild-type mice and made an atlas-based segmentation of the structures in 15 individual brains. We then performed computer automated volume measurements on the structures. We found a reduced cerebellar volume in the cdf mutants, which was expected, but we also found a new phenotype in the inferior colliculus and the olfactory bulbs. Subsequent local histology revealed additional cytoarchitectural abnormalities in the olfactory bulbs. This demonstrates the utility of anatomical magnetic resonance imaging and semiautomated image analysis for detecting abnormal neuroarchitecture in mutant mice.