Semiconductor diode laser device adjuvanting intradermal vaccine
Semiconductor diode laser device adjuvanting intradermal vaccine
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DOI：
10.1016/j.vaccine.2017.03.036
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发表时间：
2017-04-25
期刊：
影响因子：
5.5
通讯作者：
Kashiwagi, Satoshi
中科院分区：
文献类型：
--
作者：
Kimizuka, Yoshifumi;Callahan, John J.;Kashiwagi, Satoshi
皮肤短暂暴露于低功率、无组织损伤的激光已被证明可增强对皮内接种的免疫反应。临床前和临床研究均表明，与标准化学或生物佐剂相比，这种方法简单、有效、安全且耐受性良好。到目前为止，这些激光照射都是使用二极管泵浦固态激光器（DPSSL）设备进行的，这种设备价格昂贵，需要劳动密集型的维护和特殊培训。开发一种廉价、易于使用和小型的设备将是将这项技术转化为临床应用的重要一步。在这里，我们报告说，我们已经建立了一种手持式近红外（NIR）激光设备，使用半导体二极管发射1061、1258或1301 nm的光，成本不到4000美元，并且该装置在流感疫苗接种的小鼠模型中复制了DPSSL系统的佐剂作用。我们的研究结果还表明，更宽范围的近红外激光波长具有增强疫苗免疫反应的能力，允许设备设计的工程选项。这种小型，低成本的设备建立了在临床环境中使用激光佐剂方法进行大规模疫苗接种计划的可行性，为使用各种疫苗对该技术进行更广泛的测试打开了大门，并为开发可用于临床的设备奠定了基础。(C)2017爱思唯尔有限公司版权所有。
A brief exposure of skin to a low-power, non-tissue damaging laser light has been demonstrated to augment immune responses to intradermal vaccination. Both preclinical and clinical studies show that this approach is simple, effective, safe and well tolerated compared to standard chemical or biological adjuvants. Until now, these laser exposures have been performed using a diode-pumped solid-state laser (DPSSL) devices, which are expensive and require labor-intensive maintenance and special training. Development of an inexpensive, easy-to-use and small device would form an important step in translating this technology toward clinical application.Here we report that we have established a handheld, near-infrared (NIR) laser device using semiconductor diodes emitting either 1061, 1258, or 1301 nm light that costs less than $4000, and that this device replicates the adjuvant effect of a DPSSL system in a mouse model of influenza vaccination. Our results also indicate that a broader range of NIR laser wavelengths possess the ability to enhance vaccine immune responses, allowing engineering options for the device design.This small, low-cost device establishes the feasibility of using a laser adjuvant approach for mass vaccination programs in a clinical setting, opens the door for broader testing of this technology with a variety of vaccines and forms the foundation for development of devices ready for use in the clinic. (C) 2017 Elsevier Ltd. All rights reserved.