PARASOL (P-ALPHA) GANGLION-CELLS OF THE PRIMATE FOVEA - IMMUNOCYTOCHEMICAL STAINING WITH ANTIBODIES AGAINST GABA(A)-RECEPTORS
PARASOL (P-ALPHA) GANGLION-CELLS OF THE PRIMATE FOVEA - IMMUNOCYTOCHEMICAL STAINING WITH ANTIBODIES AGAINST GABA(A)-RECEPTORS
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DOI：
10.1016/0042-6989(93)90052-x
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发表时间：
1993-01-01
期刊：
影响因子：
1.8
通讯作者：
WASSLE, H
中科院分区：
文献类型：
--
作者：
GRUNERT, U;GREFERATH, U;WASSLE, H
用抗GABA（A）受体抗体对猕猴视网膜进行免疫染色。在周边视网膜神经节细胞的免疫反应。在中央视网膜，中央凹周围，神经节细胞层的染色是选择性的，只有5-8%的所有神经节细胞被标记：这些具有最大的细胞体，它们的树突占据了内丛状层中间的一个宽阔的层。从高尔基体染色的神经节细胞的比较得出结论，整个人口的阳伞（Palpha）-细胞在中央凹标记。通过结合高尔基体染色细胞的树突状场测量与免疫染色时的细胞密度，确定了梧桐细胞的镶嵌和采样特性。在每个中央凹伞神经节细胞上有30-50个视锥细胞会聚。开和关遮阳伞细胞的树突状区域提供完整的视网膜覆盖。它们的镶嵌图的奈奎斯特极限为4弧分。
Retinae of macaque monkeys were immuno-stained with antibodies against GABA(A)-receptors. In peripheral retina most ganglion cells were immunoreactive. In central retina, around the fovea, staining in the ganglion cell layer was selective and only 5-8% of all ganglion cells were labelled: these had the largest cell bodies and their dendrites occupied a broad stratum in the middle of the inner plexiform layer. From comparison with Golgi-stained ganglion cells it is concluded that the entire population of parasol (Palpha)-cells at the fovea was labelled. The mosaic and sampling properties of parasol cells were determined by combining dendritic field measurements of Golgi-stained cells with their density when immuno-stained. There is convergence of 30-50 cones onto each foveal parasol ganglion cell. The dendritic fields of both ON- and OFF-parasol cells provide complete retinal coverage. The Nyquist limits of their mosaics are 4 min of arc.