Electroacupuncture brain protection during ischemic stroke: A role for the parasympathetic nervous system
Electroacupuncture brain protection during ischemic stroke: A role for the parasympathetic nervous system
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DOI：
10.1177/0271678x17697988
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发表时间：
2018-03-01
影响因子：
6.3
通讯作者：
Li, Wenzhi
中科院分区：
文献类型：
--
作者：
Chi, Laiting;Du, Kairong;Li, Wenzhi
将副交感神经激活用于中风治疗的需求尚未得到满足。在目前的研究中，我们研究了电针(EA)在实验性中风模型中提供的神经保护是否与副交感神经系统(PNS)的激活有关。结果表明，单侧迷走神经切断联合外周阿托品所致的副交感神经功能障碍(PD)削弱了电针的功能优势及其在改善脑血流、缩小脑梗塞体积、抑制细胞凋亡、神经元和外周炎症以及氧化应激方面的作用。最重要的是，电针大鼠的胆碱乙酰转移酶、5种M受体亚型和a7nAChR的mRNA水平显著降低，提示中枢胆碱能系统的损伤受到抑制；电针还激活了迷走神经背侧运动核，迷走神经背侧运动核是下脑干副交感神经节前神经元的最大来源(c-fos免疫组织化学检测)，PD抑制了这些变化。这些发现表明，电针可作为卒中治疗中PNS激活的另一种方式。
The demand for using parasympathetic activation for stroke therapy is unmet. In the current study, we investigated whether the neuroprotection provided by electroacupuncture (EA) in an experimental stroke model was associated with activation of the parasympathetic nervous system (PNS). The results showed that parasympathetic dysfunction (PD), performed as unilateral vagotomy combined with peripheral atropine, attenuated both the functional benefits of EA and its effects in improving cerebral perfusion, reducing infarct volume, and hindering apoptosis, neuronal and peripheral inflammation, and oxidative stress. Most importantly, EA rats showed a dramatically less reduction in the mRNA level of choline acetyltransferase, five subtypes of muscarinic receptors and a7nAChR, suggesting the inhibition of the impairment of the central cholinergic system; EA also activated dorsal motor nucleus of the vagus, the largest source of parasympathetic pre-ganglionic neurons in the lower brainstem (detected by c-fos immunohistochemistry), and PD suppressed these changes. These findings indicated EA may serve as an alternative modality of PNS activation for stroke therapy.