DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function.
DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function.
复制标题
DNA 主链硫修饰通过其抗氧化功能扩大多重应力下微生物的生长范围
DOI：
10.1038/s41598-017-02445-1
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发表时间：
2017-06-14
影响因子：
4.6
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Yang Y;Xu G;Liang J;He Y;Xiong L;Li H;Bartlett D;Deng Z;Wang Z;Xiao X
DNA磷硫酰化（PT）修饰是由DndA - E蛋白在DNA骨架上引入的一种硫修饰。它已在许多细菌分离株和宏基因组数据集中被检测到，包括人类病原体，并且被认为在自然界中广泛分布。然而，对于这种修饰的生理功能知之甚少，因此其进化意义和应用潜力在很大程度上仍然是个谜。在这项研究中，我们关注DNA PT修饰对细菌细胞应对环境压力的优势。我们发现，由于DNA磷硫酰化，中温菌大肠杆菌和极端嗜压菌希瓦氏菌在暴露于极端温度、盐度、pH、压力、紫外线、X射线和重金属后，它们的生长范围都有所扩大。磷硫酰化DNA在体内对过氧化氢和羟基自由基都有反应，并保护基因组DNA以及敏感酶免受细胞内氧化损伤。我们进一步证明，这一过程与其在DNA限制和修饰中的相关作用是分开进化的。这些发现为一种在自然界中广泛存在的共价修饰提供了一种生理作用，并暗示了其在生物技术和生物医学中可能的应用。
DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins DndA-E. It has been detected within many bacteria isolates and metagenomic datasets, including human pathogens, and is considered to be widely distributed in nature. However, little is known about the physiological function of this modification, and thus its evolutionary significance and application potential remains largely a mystery. In this study, we focused on the advantages of DNA PT modification to bacterial cells coping with environmental stresses. We show that the mesophile Escherichia coli and the extremophile Shewanella piezotolerans both expanded their growth ranges following exposure to extreme temperature, salinity, pH, pressure, UV, X-ray and heavy metals as a result of DNA phophorothioation. The phophorothioated DNA reacted to both H2O2 and hydroxyl radicals in vivo, and protected genomic DNA as well as sensitive enzymes from intracellular oxidative damage. We further demonstrate that this process has evolved separate from its associated role in DNA restriction and modification. These findings provide a physiological role for a covalent modification widespread in nature and suggest possible applications in biotechnology and biomedicine.