Selective translocation of protein kinase C-delta in PC12 cells during nerve growth factor-induced neuritogenesis.
Selective translocation of protein kinase C-delta in PC12 cells during nerve growth factor-induced neuritogenesis.
复制标题
神经生长因子诱导的神经突发生过程中 PC12 细胞中蛋白激酶 C-δ 的选择性易位。
DOI：
10.1091/mbc.6.4.449
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发表时间：
1995
影响因子：
3.3
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
O'Driscoll,KR;Teng,KK;Fabbro,D;Greene,LA;Weinstein,IB
神经生长因子（NGF）在PC12大鼠嗜铬细胞瘤细胞中引发神经突形成的特异性细胞内信号尚不清楚。在神经生长因子诱导的神经发生过程中，蛋白激酶c - δ (PKC δ)从可溶性组分转移到颗粒亚细胞组分；然而，在表皮生长因子治疗后，这种情况不会发生，表皮生长因子是有丝分裂的，但不会诱导神经突的形成。PC12细胞还含有Ca（2+）敏感和Ca（2+）不依赖的PKC酶活性，并表达与PKC亚型α、β、δ、epsilon和zeta相对应的mRNA和免疫反应蛋白。NGF治疗1-3天后，免疫反应性PKC α、β和epsilon水平出现短暂性下降，7天后，PKC α水平升高2.5倍，细胞总PKC活性升高1.8倍。ngf诱导的PC12细胞的神经新生在TPA剂量和时间依赖性的作用下得到增强，这种分化与细胞在TPA作用下PKC δ和其他PKC亚型下调的消除一致。因此，PKC δ的选择性激活可能在PC12细胞的神经源性信号中发挥作用。
The specific intracellular signals initiated by nerve growth factor (NGF) that lead to neurite formation in PC12 rat pheochromocytoma cells are as of yet unclear. Protein kinase C-delta (PKC delta) is translocated from the soluble to the particulate subcellular fraction during NGF-induced-neuritogenesis; however, this does not occur after treatment with the epidermal growth factor, which is mitogenic but does not induce neurite formation. PC12 cells also contain both Ca(2+)-sensitive and Ca(2+)-independent PKC enzymatic activities, and express mRNA and immunoreactive proteins corresponding to the PKC isoforms alpha, beta, delta, epsilon, and zeta. There are transient decreases in the levels of immunoreactive PKCs alpha, beta, and epsilon after 1-3 days of NGF treatment, and after 7 days there is a 2.5-fold increase in the level of PKC alpha, and a 1.8-fold increase in total cellular PKC activity. NGF-induced PC12 cell neuritogenesis is enhanced by 12-O-tetradecanoyl phorbol-13-acetate (TPA) in a TPA dose- and time-dependent manner, and this differentiation coincides with abrogation of the down-regulation of PKC delta and other PKC isoforms, when the cells are treated with TPA. Thus a selective activation of PKC delta may play a role in neuritogenic signals in PC12 cells.