Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm, Spodoptera exigua (Hubner)
Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm, Spodoptera exigua (Hubner)
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甜菜夜蛾、甜菜夜蛾 (Hubner) 对转基因 Bt 棉花代谢耐受性的时间分配
DOI：
10.1007/s11427-010-4133-y
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发表时间：
2011-02-01
影响因子：
9.1
通讯作者：
Wan FangHao
中科院分区：
文献类型：
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作者：
Guo JianYing;Wu Gang;Wan FangHao
以转基因苏云金芽孢杆菌（Bt）和非Bt棉花为食，对四龄甜菜军虫Spodoptera exigua （Hubner）体内消化解毒酶活性和营养成分进行了研究。棉花的营养成分和特定酶活性在Bt棉和非Bt棉之间存在显著差异。在1、6和24 h，饲喂Bt棉花的黑穗槐游离脂肪酸和葡萄糖水平显著低于饲喂非Bt棉花的黑穗槐。在饲喂Bt棉花的各采食时段，棉铃虫胰蛋白酶和总超氧化物歧化酶（T-SOD）活性显著高于未饲喂Bt棉花的棉铃虫，而脂肪酶、羧酸酯酶和乙酰胆碱酯酶活性显著低于未饲喂Bt棉花的棉铃虫。各棉花品种组取食次数也存在差异。采食1 h的赤眼蜂游离脂肪酸和总氨基酸显著低于采食1 h的赤眼蜂，采食24 h的赤眼蜂脂肪酶和胰蛋白酶活性显著低于采食1和4 h的赤眼蜂，而采食24 h的赤眼蜂羧酸酯酶和乙酰胆碱酯酶活性显著高于采食1 h的赤眼蜂。棉花品种与采食时间的交互作用显著影响了棉花脂酶、胰蛋白酶、乙酰胆碱酯酶和T-SOD酶的活性。在苏云金芽孢杆菌的胁迫下，通过测定其体内保护和解毒酶活性的时间分配，可以对草食性昆虫在有毒蛋白的持续选择压力下的代谢耐受性进行有意义的评价。
Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet army-worms, Spodoptera exigua (Hubner), fed on transgenic Bacillus thuringiensis (Bt) and non-Bt cotton for different time periods. Nutrient composition and specific enzyme activities differed significantly between the S. exigua fed Bt vs. non-Bt cotton. At 1, 6 and 24 h, free fatty acid and glucose levels were significantly lower in S. exigua fed on Bt cotton than those fed on non-Bt cotton. S. exigua fed on Bt cotton had significantly higher trypsin and total superoxide dismutase (T-SOD) activities and significantly lower lipase, carboxylesterase and acetylcholinesterase activities than non-Bt fed worms for all feeding time periods. Differences were also observed among feeding times within each cotton variety group. Significantly lower free fatty acid and total amino acid were observed in S. exigua fed on Bt cotton for 24 h than in those fed for 1 h. Significantly lower activities of lipase and trypsin were detected in S. exigua fed on Bt cotton for 24 h than those for 1 and 4 h. However, carboxylesterase and acetylcholinesterase activities in S. exigua fed on Bt cotton for 24 h were significantly higher than those for 1, 4 and 6 h. The interaction between cotton variety and feeding time significantly affected the activities of lipase, trypsin, acetylcholinesterase and T-SOD enzymes in S. exigua. Measuring the temporal allocation of protection and detoxification enzyme activities in the body of S. exigua in response to B. thuringiensis can provide a meaningful evaluation on the metabolic tolerance of herbivorous insects under the continuous selection pressure of a toxic protein.