Azithromycin-carrying and microtubule-orientated biomimetic poly (lactic-co-glycolic acid) scaffolds for eyelid reconstruction.
Azithromycin-carrying and microtubule-orientated biomimetic poly (lactic-co-glycolic acid) scaffolds for eyelid reconstruction.
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DOI：
10.3389/fmed.2023.1129606
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发表时间：
2023
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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睑板修复是眼部整形外科医生眼睑重建的主要挑战。理想的合成睑板替代品应模仿自然眼睑的微观结构和机械强度。这项工作的目的是开发一种用于眼睑重建的新型仿生替代品。使用改进的热诱导相分离技术制备了三种类型的聚乳酸-乙醇酸 (PLGA) 支架（随机支架、定向支架和负载阿奇霉素的定向支架）。通过扫描电子显微镜检查支架的微观结构。使用支架提取物评估体外细胞毒性。在支架内培养成纤维细胞和原代大鼠睑板腺上皮细胞（rMGC），并使用荧光染色观察它们的行为。将三种类型的PLGA支架植入兔眼睑缺损体内，评价其诱导组织修复功能。我们成功制造了三种具有不同孔结构的 PLGA 支架，并且轴向对齐的支架表现出互连和垂直平行的通道。使用支架提取物的体外细胞毒性测试显示没有明显的细胞毒性。荧光染色显示成纤维细胞和rMGCs都能很好地粘附在孔壁上，成纤维细胞沿着轴向排列的多孔结构伸长。植入后 8 周，所有支架均通过纤维血管组织良好整合。与随机支架组相比，轴向对齐的支架组表现出更快的降解，更小的碎片被成熟的胶原纤维包围。研究发现，轴向排列的支架可以很好地支持和引导体外和体内的细胞活动。此外，与随机支架组相比，轴向对齐支架组表现出更快的降解速率和匹配的整合速率。研究结果表明，定向支架是眼睑睑板替代品的一个有前途的替代品。
Tarsal plate repair is the major challenge of eyelid reconstruction for the oculoplastic surgeon. The ideal synthetic tarsal plate substitute should imitate the microstructure and mechanical strength of the natural eyelid. The aim of this work was to develop a novel bionic substitute for eyelid reconstruction. Three types of poly(lactic-co-glycolic acid) (PLGA) scaffolds (random, oriented, and azithromycin-loaded oriented scaffolds) were prepared using an improved thermal-induced phase separation technique. The microstructure of the scaffolds was examined by scanning electron microscopy. In vitro cytotoxicity was assessed using scaffold extracts. Fibroblast and primary rat meibomian gland epithelial cells (rMGCs) were cultured within the scaffolds, and their behavior was observed using fluorescence staining. Three types of PLGA scaffolds were implanted into rabbit eyelid defect in vivo to evaluate their inductive tissue repair function. We successfully fabricated three types of PLGA scaffolds with varying pore architectures, and the axially aligned scaffold demonstrated interconnected and vertically parallel channels. In vitro cytotoxicity tests using scaffold extracts revealed no apparent cytotoxicity. Fluorescence staining showed that both Fibroblast and rMGCs could adhere well onto the pore walls, with fibroblast elongating along the axially aligned porous structure. At 8 weeks post-implantation, all scaffolds were well integrated by fibrovascular tissue. The axially aligned scaffold groups exhibited faster degradation compared to the random scaffold group, with smaller fragments surrounded by mature collagen fibers. The study found that the axially aligned scaffolds could well support and guide cellular activities in vitro and in vivo. Moreover, the axially aligned scaffold group showed a faster degradation rate with a matched integration rate compared to the random scaffold group. The findings suggest that the oriented scaffold is a promising alternative for eyelid tarsal plate substitutes.
DOI：
10.5500/wjt.v12.i7.175
发表时间：
2022-07-18
期刊：
World journal of transplantation
影响因子：
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作者：
Miotti, Giovanni;Zeppieri, Marco;Rodda, Agostino;Salati, Carlo;Parodi, Pier Camillo
通讯作者：
Parodi, Pier Camillo