Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: A pilot study
Exhaled breath condensate eicosanoids and sputum eosinophils in asthmatic children: A pilot study
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DOI：
10.1046/j.0905-6157.2003.00097.x
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发表时间：
2004-02-01
影响因子：
4.4
通讯作者：
Piacentini, GL
中科院分区：
文献类型：
--
作者：
Bodini, A;Peroni, D;Piacentini, GL
哮喘患儿呼出气冷凝液（EBC）中半胱氨酰白三烯（cys-LTs）、LTB_4和8-异前列腺素（8-isoprostane）水平升高。本研究旨在探讨测定EBC中cys-LTs、LTB_4和8-异前列腺素水平是否能反映哮喘患儿在自然回避屋尘螨（HDM）过敏原的过程中诱导痰中气道炎症的水平。12名儿童在入院时（TO）和3个月后（T1）在Istituto Pio XII（Misurina，意大利白云岩1756米）进行了评估。评价T0和T1时痰液嗜酸性粒细胞百分比和呼吸冷凝液中cys-LT、LTB 4和8-异前列腺素的测量值。T0和T1时诱导痰中嗜酸性粒细胞百分比分别为8.5 +/- 1.1%和3.5 +/- 0.4%（p = 0.011）。T0时痰液中的中性粒细胞百分比为1.1 +/- 0.5%，T1时为1.5 +/- 1.0%（ns）。T0时Cys-LTs平均水平为14.24 +/- 4.53 pg/ml，T1时为4.65 +/- 0.68 pg/ml（p = 0.0125）。T0时LTB 4水平为2.36 +/- 0.19 pg/ml，T1时为2.41 +/- 0.23 pg/ml（ns）。8-异前列烷水平从T0时的17.47 +/- 3.18 pg/ml降至T1时的7.36 +/- 3.26 pg/ml（p = 0.003）。本研究表明，呼出cys-LTs和8-异前列烷，以及诱导痰中嗜酸性粒细胞的百分比，减少过敏原回避后，哮喘儿童提示EBC的潜在应用，为无创评估过敏性哮喘儿童的气道炎症。
Cysteinyl leukotrienes (cys-LTs), LTB4 and 8-isoprostane are increased in the exhaled breath condensate (EBC) from asthmatic patients The aim of this study was to investigate whether the measurement of cys-LTs, LTB4 and 8-isoprostane in EBC can reflect the level of airway inflammation assessed by induced sputum in asthmatic children sensitized to house dust mite (HDM) during natural avoidance of HDM allergens. Twelve children were evaluated at the time of admission (TO) and after 3 months of stay (T1) at the Istituto Pio XII (Misurina, Italian Dolomites 1756 m). Sputum eosinophil percentage and measurement of cys-LTs, LTB4 and 8-isoprostanes in the breath condensate at T0 and T1 were evaluated. Eosinophil percentage in induced sputum was 8.5 +/- 1.1% at T0 and 3.5 +/- 0.4% at T1 (p = 0.011). Neutrophil percentage in sputum was 1.1 +/- 0.5% at T0 and 1.5 +/- 1.0% at T1 (ns). Cys-LTs mean level was 14.24 +/- 4.53 pg/ml at T0 and 4.65 +/- 0.68 pg/ml at T1 (p = 0.0125). LTB4 level was 2.36 +/- 0.19 pg/ml at T0 and 2.41 +/- 0.23 pg/ml at T1 (ns). 8-Isoprostane level reduced from 17.47 +/- 3.18 pg/ml at T0 to 7.36 +/- 3.26 pg/ml at T1 (p = 0.003). This study show that exhaled cys-LTs and 8-isoprostane, as well as eosinophil percentage in induced sputum, are reduced after allergen avoidance in asthmatic children suggesting a potential application of EBC for the non-invasive evaluation of airway inflammation in asthma in allergic asthmatic children.