FLP/FRT and Cre/lox recombination technology in C. elegans.
FLP/FRT and Cre/lox recombination technology in C. elegans.
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DOI：
10.1016/j.ymeth.2014.05.007
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发表时间：
2014-08-01
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影响因子：
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通讯作者：
Hubbard EJ
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作者：
Hubbard EJ
利用模式生物进行生物学研究的最有力的方面之一是控制基因表达的能力。圣杯是对特定基因产物表达的时间和空间控制--也就是说，在特定时间在特定细胞中表达或抑制基因或其产物活性的能力。理想情况下，这种方法还可以调节基因活性的精确水平，并且改变是可逆的。可见标志物的受控或有目的地随机化表达的相关目标也是非常强大的。虽然并非所有这些壮举都是在C中完成的。到目前为止，已经取得了很大的进展，最近的技术使这些目标更加接近。在这里，我提出了成功的双组分位点特异性重组在C。优美的这些技术基于由FLP或Cre重组酶驱动的在限定位点之间的DNA序列的受控分子内切除或倒置的原理。我讨论了这项技术的未来应用的几个前景。
One of the most powerful aspects of biological inquiry using model organisms is the ability to control gene expression. A holy grail is both temporal and spatial control of the expression of specific gene products – that is, the ability to express or withhold the activity of genes or their products in specific cells at specific times. Ideally such a method would also regulate the precise levels of gene activity, and alterations would be reversible. The related goal of controlled or purposefully randomized expression of visible markers is also tremendously powerful. While not all of these feats have been accomplished in C. elegans to date, much progress has been made, and recent technologies put these goals within closer reach. Here, I present published examples of successful two-component site-specific recombination in C. elegans. These technologies are based on the principle of controlled intra-molecular excision or inversion of DNA sequences between defined sites, as driven by FLP or Cre recombinases. I discuss several prospects for future applications of this technology.