HUMAN BRONCHIAL EPITHELIAL-CELLS WITH INTEGRATED SV40 VIRUS T-ANTIGEN GENES RETAIN THE ABILITY TO UNDERGO SQUAMOUS DIFFERENTIATION
HUMAN BRONCHIAL EPITHELIAL-CELLS WITH INTEGRATED SV40 VIRUS T-ANTIGEN GENES RETAIN THE ABILITY TO UNDERGO SQUAMOUS DIFFERENTIATION
复制标题
DOI：
10.1111/j.1432-0436.1988.tb00592.x
复制
发表时间：
1988-01-01
期刊：
影响因子：
2.9
通讯作者：
HARRIS, CC
中科院分区：
文献类型：
--
作者：
KE, Y;REDDEL, RR;HARRIS, CC
研究了DNA病毒感染（SV40或腺病毒12-SV40杂交病毒）或转染SV40大T抗原基因转化的人支气管上皮细胞在暴露于12- o - tetradecanoylphorbol13 acetate (TPA)，转化生长因子-. β 1时发生鳞状分化的能力(TGF度量。1)，或胎牛血清（FBS），诱导正常人支气管上皮细胞鳞状分化的药物。当暴露于FBS或TGF- β时，所有10个t抗原阳性细胞培养物均发生鳞状分化。1，但暴露于TPA时没有分化。从一个被命名为BEAS-2B的细胞系中分离出两个亚克隆，其中一个被FBS诱导成鳞状分化，另一个未发生鳞状分化，并在暴露于血清时刺激有丝分裂。这些表型不同的亚克隆为描述人支气管上皮细胞在FBS或TGF-. β作用下的鳞状分化机制提供了一个新的体外细胞系统。
Human bronchial epithelial cells transformed by either DNA virus infection (SV40 or Adenovirus 12-SV40 hybrid virus) or transfection with the SV40 large T antigen gene were studied for their ability to undergo squamous differentiation when exposed to 12-O-tetradecanoylphorbol-13 acetate (TPA), transforming growth factor-.beta.1 (TGF-.beta.1), or fetal bovine serum (FBS), agents that induce the squamous differentiation of normal human bronchial epithelial cells. Squamous differentiation occurred in all ten T-antigen-positive cell cultures when they were exposed to either FBS or TGF-.beta.1, but none differentiated when exposed to TPA. From one cell line, designated BEAS-2B, two subclones were isolated, one of which was induced to undergo squamous differentiation by FBS, and a second that failed to undergo squamous differentiation and was mitogenically stimulated when exposed to serum. These phenotypically different subclones provide a new vitro cellular system for delineating the mechanism(s) of human bronchial epithelial cell squamous differentiation in response to FBS or TGF-.beta.1.