European bat Lyssavirus infection in Spanish bat populations
European bat Lyssavirus infection in Spanish bat populations
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DOI：
10.3201/eid0804.010263
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发表时间：
2002-04-01
影响因子：
11.8
通讯作者：
Bourhy, H
中科院分区：
文献类型：
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作者：
Serra-Cobo, J;Amengual, B;Bourhy, H
从1992年到2000年，从西班牙37个地点的14种蝙蝠中获得了976份血清、27个血团和91个大脑。在阿拉贡和巴利阿里群岛地区的鼠耳蝠、长翼蝠、Tadarida teniotis和Rhinolophus ferrumequinum中检测到特异性抗欧洲蝙蝠狂犬病毒1型（EBL 1）中和抗体。巢式逆转录-聚合酶链反应（RT-PCR）在脑、血颗粒、肺、心、舌、食管-咽喉等组织中也获得阳性结果。鼠耳蝠（Myotis nattereri）、鼠耳蝠R. ferrumequinum和M. schreibersii。核苷酸序列的测定证实了EBL 1 RNA在不同组织中的存在。在一个殖民地，血清阳性蝙蝠的患病率随着时间的推移对应于一个不对称的曲线，突然初始增加峰值在60%的蝙蝠，然后逐渐下降。带状血清反应阳性蝙蝠在几年内恢复，表明EBL 1感染这些蝙蝠是非致命的。该物种中至少有一种（M。schreibersii）是迁徙性的，因此可能是EBL 1在地中海两岸传播的部分原因。
From 1992 to 2000, 976 sera, 27 blood pellets, and 91 brains were obtained from 14 bat species in 37 localities in Spain. Specific anti-European bat lyssavirus 1 (EBL1)-neutralizing antibodies have been detected in Myotis myotis, Miniopterus schrelbersii, Tadarida teniotis, and Rhinolophus ferrumequinum in the region of Aragon and the Balearic Islands. Positive results were also obtained by nested reverse transcription-polymerise chain reaction on brain, blood pellet, lung, heart, tongue, and esophagus-larynx-pharynx of M. myotis, Myotis nattereri, R. ferrumequinum, and M. schreibersii. Determination of nucleotide sequence confirmed the presence of EBL1 RNA in the different tissues. In one colony, the prevalence of seropositive bats over time corresponded to an asymmetrical curve, with a sudden initial increase peaking at 60% of the bats, followed by a gradual decline. Banded seropositive bats were recovered during several years, indicating that EBL1 infection in these bats was nonlethal. At least one of this species (M. schreibersii) is migratory and thus could be partially responsible for the dissemination of EBL1 on both shores of the Mediterranean Sea.